Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma
| العنوان: | Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma |
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| المؤلفون: | Spartaco Santi, Barbara Dozza, Enrico Lucarelli, A. Guerrini, Paolo Dambruoso, Davide Maria Donati, Giovanna Sotgiu, Claudia Ferroni, Marco Ballestri, Greta Varchi, Sandro Giannini, Serena Duchi |
| المساهمون: | Duchi S, Sotgiu G, Lucarelli E, Ballestri M, Dozza B, Santi S, Guerrini A, Dambruoso P, Giannini S, Donati D, Ferroni C, Varchi G |
| المصدر: | Journal of controlled release 168 (2013): 225–237. doi:10.1016/j.jconrel.2013.03.012 info:cnr-pdr/source/autori:Duchi, Serena; Sotgiu, Giovanna; Lucarelli, Enrico; Ballestri, Marco; Dozza, Barbara; Santi, Spartaco; Guerrini, Andrea; Dambruoso, Paolo; Giannini, Sandro; Donati, Davide Maria; Ferroni, Claudia; Varchi, Greta/titolo:Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma/doi:10.1016%2Fj.jconrel.2013.03.012/rivista:Journal of controlled release/anno:2013/pagina_da:225/pagina_a:237/intervallo_pagine:225–237/volume:168 |
| بيانات النشر: | Elsevier BV, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | bone marrow, Porphyrins, Stromal cell, Cell Survival, Mesenchymal stem cells (MSC), Photodynamic therapy (PDT), medicine.medical_treatment, Pharmaceutical Science, Apoptosis, Bone Neoplasms, Photodynamic therapy, Nanotechnology, Osteosarcoma (OS), law.invention, PHOTODYNAMIC THERAPY, Drug Delivery Systems, Confocal microscopy, law, Cell Line, Tumor, medicine, cancer, Humans, Photosensitizer, Osteosarcoma, Photosensitizing Agents, Chemistry, Nanoparticles (NPs), Mesenchymal stem cell, Porphyrins (TPPS), Mesenchymal Stem Cells, CHEMOTHERAPY, Doxorubicin, In vitro, DRUG RESISTANCE, Photochemotherapy, Cell culture, mesenchymal stromal cell, Cancer research, Nanoparticles, Reactive Oxygen Species |
| الوصف: | Mesenchymal stem cells (MSC) have the unique ability to home and engraft in tumor stroma. These features render them potentially a very useful tool as targeted delivery vehicles which can deliver therapeutic drugs to the tumor stroma. In the present study, we investigate whether fluorescent core-shell PMMA nanoparticles (FNPs) post-loaded with a photosensitizer, namely meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) and uploaded by MSC could trigger osteosarcoma (OS) cell death in vitro upon specific photoactivation. In co-culture studies we demonstrate using laser confocal microscopy and time lapse imaging, that only after laser irradiation MSC loaded with photosensitizer- coated fluorescent NPs (TPPS@FNPs) undergo cell death and release reactive oxygen species (ROS) which are sufficient to trigger cell death of all OS cells in the culture. These results encourage further studies aimed at proving the efficacy of this novel tri-component system for PDT applications. © 2013 Elsevier B.V. |
| وصف الملف: | STAMPA |
| تدمد: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2013.03.012 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed8be299ba3872b1eb24c76fdcc4ef9d https://doi.org/10.1016/j.jconrel.2013.03.012 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....ed8be299ba3872b1eb24c76fdcc4ef9d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01683659 |
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| DOI: | 10.1016/j.jconrel.2013.03.012 |