Bone formation induced by BMP-2 in human osteosarcoma cells
| العنوان: | Bone formation induced by BMP-2 in human osteosarcoma cells |
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| المؤلفون: | Frank La Marca, Khoi D. Than, Lin Wang, Chia-Ying Lin, Shayan U. Rahman, Paul Park |
| المصدر: | International Journal of Oncology |
| بيانات النشر: | Spandidos Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, Cell, osteogenic differentiation, Bone Morphogenetic Protein 2, Aldehyde dehydrogenase, Bone Morphogenetic Protein Receptors, Type II, Bone morphogenetic protein 2, Mice, 03 medical and health sciences, 0302 clinical medicine, Bone Density, Osteogenesis, Cancer stem cell, BMP-2, Cell Line, Tumor, medicine, Animals, Humans, Bone Morphogenetic Protein Receptors, Type I, Smad, bone formation, 030304 developmental biology, Osteosarcoma, 0303 health sciences, biology, Articles, Aldehyde Dehydrogenase, Flow Cytometry, medicine.disease, MAPK, Molecular biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Apoptosis, Cell culture, 030220 oncology & carcinogenesis, biology.protein, Alkaline phosphatase |
| الوصف: | Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH(br) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH(br) and non-tumorigenic ALDH(lo) cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo. There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDH(lo) cells compared with that in ALDH(br) cells and the BMPR1B expression in ALDH(lo) cells was ~8-fold higher compared to that in ALDHbr cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDH(lo) cells compared to ALDH(br) cells, which were mediated by the canonical Smad signaling pathway. In vivo, BMP-2 was identified to induce bone formation in both ALDH(br) and ALDH(lo) cells. All animals receiving 1 x 10()4 ALDH(lo) cells treated with 30 µg of BMP-2 per animal showed bone formation within 1-2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDH(lo) cells showed significantly more bone mineral content compared to that in ALDH(br) cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway. |
| تدمد: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2013.2030 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed15148d5ae13e8c4e367b4ff1263989 https://doi.org/10.3892/ijo.2013.2030 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ed15148d5ae13e8c4e367b4ff1263989 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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| DOI: | 10.3892/ijo.2013.2030 |