Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway
| العنوان: | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway |
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| المؤلفون: | Xueyun Liang, Yan Wang, Jiaxin Liu, Baocong Yu, Yiran Jin, Jiahui Li, Xiaona Ma, Jianqiang Yu, Jianguo Niu |
| المصدر: | Molecular Biology Reports. 49:7337-7345 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Neurons, Oxidative Stress, MAP Kinase Signaling System, Culture Media, Conditioned, Genetics, TRPM Cation Channels, Mesenchymal Stem Cells, Hydrogen Peroxide, General Medicine, Molecular Biology, Umbilical Cord |
| الوصف: | Background The mechanism by which MSC-CM protects neuronal cells against ischemic injury remains to be elucidated. In this study, we aimed to clarify the protective effect of umbilical cord-derived mesenchymal stem cell conditioned medium (UC-MSC-CM) on neuronal oxidative injury and its potential mechanism. Methods and Results Neuronal oxidative damage was mimicked by H2O2 treatment of the HT22 cell line. The numbers of cleaved-Caspase-3-positive cells and protein expression of Caspase-9 induced by H2O2 treatment were decreased by UC-MSC-CM treatment. Furthermore, SOD protein expression was increased in the MSC-CM group compared with that in the H2O2 group. The H2O2-induced TRPM2-like currents in HT22 cells were attenuated by MSC-CM treatment. In addition, H2O2 treatment downregulated the expression of p-JNK protein in HT22 cells, and this the downward trend was reversed by incubation with MSC-CM. Conclusions UC-MSC-CM protects neurons against oxidative injury, possibly by inhibiting activation of TRPM2 and the JNK signaling pathway. |
| تدمد: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-022-07524-9 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecfc2a1dc923e94199ffbbe64327248c https://doi.org/10.1007/s11033-022-07524-9 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ecfc2a1dc923e94199ffbbe64327248c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734978 03014851 |
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| DOI: | 10.1007/s11033-022-07524-9 |