Epigenetic Modification Affecting Expression of Cell Polarity and Cell Fate Genes to Regulate Lineage Specification in the Early Mouse Embryo
| العنوان: | Epigenetic Modification Affecting Expression of Cell Polarity and Cell Fate Genes to Regulate Lineage Specification in the Early Mouse Embryo |
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| المؤلفون: | Magdalena Zernicka-Goetz, David-Emlyn Parfitt |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Blastomeres, Protein-Arginine N-Methyltransferases, Cell Physiology, Cell division, Cellular differentiation, Cell, Down-Regulation, Cell Cycle Proteins, Biology, Cell fate determination, Epigenesis, Genetic, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Cell polarity, medicine, Animals, Inner cell mass, CDX2 Transcription Factor, Cell Lineage, RNA, Messenger, Cell Cycle Protein, Molecular Biology, Protein Kinase C, Adaptor Proteins, Signal Transducing, 030304 developmental biology, Homeodomain Proteins, Regulation of gene expression, 0303 health sciences, Cell Polarity, Gene Expression Regulation, Developmental, Articles, Cell Biology, Embryo, Mammalian, Cell biology, Mice, Inbred C57BL, Protein Transport, medicine.anatomical_structure, Cell Adhesion Molecules, Cell Division, 030217 neurology & neurosurgery, Transcription Factors |
| الوصف: | In this study, cell behavior and blastomere polarity are examined in the context of Carm1 overexpression in the preimplantation mouse embryo. The results suggest that Carm1 levels can affect the expression of key cell polarity and fate-determining in association with changes in cell behavior and lineage allocation. Formation of inner and outer cells of the mouse embryo distinguishes pluripotent inner cell mass (ICM) from differentiating trophectoderm (TE). Carm1, which methylates histone H3R17 and R26, directs cells to ICM rather that TE. To understand the mechanism by which this epigenetic modification directs cell fate, we generated embryos with in vivo–labeled cells of different Carm1 levels, using time-lapse imaging to reveal dynamics of their behavior, and related this to cell polarization. This shows that Carm1 affects cell fate by promoting asymmetric divisions, that direct one daughter cell inside, and cell engulfment, where neighboring cells with lower Carm1 levels compete for outside positions. This is associated with changes to the expression pattern and spatial distribution of cell polarity proteins: Cells with higher Carm1 levels show reduced expression and apical localization of Par3 and a dramatic increase in expression of PKCII, antagonist of the apical protein aPKC. Expression and basolateral localization of the mouse Par1 homologue, EMK1, increases concomitantly. Increased Carm1 also reduces Cdx2 expression, a transcription factor key for TE differentiation. These results demonstrate how the extent of a specific epigenetic modification could affect expression of cell polarity and fate-determining genes to ensure lineage allocation in the mouse embryo. |
| وصف الملف: | application/pdf |
| تدمد: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e10-01-0053 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecea1e5e698376264f2a1f167bdee879 https://doi.org/10.1091/mbc.e10-01-0053 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ecea1e5e698376264f2a1f167bdee879 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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| DOI: | 10.1091/mbc.e10-01-0053 |