The Air Liquid-interface, a Skin Microenvironment, Promotes Growth of Melanoma Cells, but not Their Apoptosis and Invasion, through Activation of Mitogen-activated Protein Kinase
| العنوان: | The Air Liquid-interface, a Skin Microenvironment, Promotes Growth of Melanoma Cells, but not Their Apoptosis and Invasion, through Activation of Mitogen-activated Protein Kinase |
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| المؤلفون: | Nobuhisa Yonemitsu, Aki Matsunobu, Uemura Tetsuji, Shuji Toda, Meihua Piao, Noriyuki Misago, Hajime Sugihara, Fumio Yamasaki, Kazuyoshi Uchihashi, Chong Hong Yee, Shigehisa Aoki |
| المصدر: | Acta Histochemica et Cytochemica |
| بيانات النشر: | Japan Society of Histochemistry and Cytochemistry, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Pathology, medicine.medical_specialty, Histology, mitogen-activated protein kinase, Physiology, animal diseases, proliferation, collagen gel invasion assay, Cell, air-liquid interface, Biochemistry, Pathology and Forensic Medicine, chemistry.chemical_compound, melanoma, Medicine, Protein kinase A, business.industry, Cell growth, MEK inhibitor, Melanoma, Regular Article, Cell Biology, respiratory system, medicine.disease, respiratory tract diseases, medicine.anatomical_structure, chemistry, Apoptosis, Cancer research, business, Bromodeoxyuridine |
| الوصف: | The air-liquid interface (ALI) is a common microenvironment of the skin, but it is unknown whether the ALI affects melanoma cell behaviors. Using a collagen gel invasion assay, immunohistochemistry, and Western blots, here we show that melanoma cell proliferation in cultures with an ALI is higher than melanoma cell proliferation in submerged cultures. Bromodeoxyuridine (BrdU) uptake, an indicator of cell proliferation, of melanoma cells at the ALI was about 3 times that of submerged cells, while ALI and submerged melanoma cells had similar levels of single-stranded DNA (a marker of apoptosis). The ALI enhanced the expression of Raf-1, MEK-1 and pERK-1/2 components of the mitogen-activated protein kinase (MAPK) cascade, in cells more than the submerged condition did. The increases in BrdU uptake and pERK-1/2 expression promoted by ALI was abolished by the MEK inhibitor, PD-98059. ALI-treated and submerged melanoma cells did not infiltrate into the collagen gel, and they showed no significant difference in the expression of the invasion- and motility-related molecules, matrix metalloproteinase-1 and -9, laminin 5, and filamin A. Our data indicate that the ALI, a skin microenvironment, accelerates the growth, but not the apoptosis or invasion, of melanoma cells through MAPK activation. |
| اللغة: | English |
| تدمد: | 1347-5800 0044-5991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebd991019bce2491055e07f945ac5e14 http://europepmc.org/articles/PMC2840220 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebd991019bce2491055e07f945ac5e14 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475800 00445991 |
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