التفاصيل البيبلوغرافية
| العنوان: |
Polycomb Regulates Mesoderm Cell Fate-Specification in Embryonic Stem Cells through Activation and Repression Mechanisms |
| المؤلفون: |
Luigi Aloia, Alexandra Santanach, Enrique Blanco, Luciano Di Croce, Elphège P. Nora, Lluis Morey, Benoit G. Bruneau |
| المصدر: |
Recercat. Dipósit de la Recerca de Catalunya
instname |
| بيانات النشر: |
Elsevier Inc. |
| مصطلحات موضوعية: |
Mesoderm, Transcription, Genetic, Cellular differentiation, Polycomb-Group Proteins, Embryoid body, macromolecular substances, Cell fate determination, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Regulació genètica, Genetics, medicine, Animals, Mesoderm Cell, Cell Lineage, Myocytes, Cardiac, Embryoid Bodies, 030304 developmental biology, Polycomb Repressive Complex 1, 0303 health sciences, Genome, Protein Stability, Myocardium, Cell Differentiation, Mouse Embryonic Stem Cells, Cell Biology, Embryonic stem cell, Cell biology, Cartilage, medicine.anatomical_structure, Gene Expression Regulation, Bone Morphogenetic Proteins, Molecular Medicine, Diferenciació cel·lular, NODAL, 030217 neurology & neurosurgery, Protein Binding, Signal Transduction, Adult stem cell |
| الوصف: |
Polycomb complexes (PRC1 and PRC2) are essential regulators of epigenetic gene silencing in embryonic and adult stem cells. Emerging evidence suggests that the core subunit composition regulates distinct biological processes, yet little is known about the mechanistic underpinnings of how differently composed Polycomb complexes instruct and maintain cell fate. Here we find that Mel18, also known as Pcgf2 and one of six Pcgf paralogs, uniquely regulates PRC1 to specify mesoderm cell fate in embryonic stem cells. Mechanistically, Mel18 functions as a classical Polycomb protein during early cardiac mesoderm differentiation by repressing pluripotency, lineage specification, late cardiac differentiation, and negative regulators of the BMP pathway. However, Mel18 also positively regulates expression of key mesoderm transcription factors, revealing an unexpected function of Mel18 in gene activation during cardiac differentiation. Taken together, our findings reveal that Mel18 is required to specify PRC1 function in both a context- and stage-specific manner. This work was supported by grants from the Spanish “Ministerio de Educación y Ciencia” (SAF2013-48926-P), from AGAUR, from Fundació “La Marató de TV3”, and from the European Commission’s 7th Framework Program 4DCellFate (277899) |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1934-5909 |
| DOI: |
10.1016/j.stem.2015.08.009 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e94e55b3a29471fd967e56b56d855fbf |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....e94e55b3a29471fd967e56b56d855fbf |
| قاعدة البيانات: |
OpenAIRE |