Enhanced universal quantification of biomolecules using element MS and generic standards: application to intact protein and phos-phoprotein determination
| العنوان: | Enhanced universal quantification of biomolecules using element MS and generic standards: application to intact protein and phos-phoprotein determination |
|---|---|
| المؤلفون: | Silvia Diez-Fernández, Jorge Ruiz Encinar, Juan J. Calvete, Tetsushi Sakai, Michiko Yamanaka, Alfredo Sanz-Medel, Naoki Sugiyama, Francisco Calderón-Celis |
| المساهمون: | Ministerio de Economía y Competitividad (España) |
| المصدر: | RUO. Repositorio Institucional de la Universidad de Oviedo instname Digital.CSIC. Repositorio Institucional del CSIC |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Models, Molecular, Accuracy and precision, Signal response, Chromatography, Venoms, Biomolecule, Absolute quantification, 010401 analytical chemistry, Intact protein, 010402 general chemistry, Phosphoproteins, 01 natural sciences, Mass Spectrometry, 0104 chemical sciences, Analytical Chemistry, Universal quantification, chemistry, Phosphoprotein, Absolute protein quantification, Chromatography, High Pressure Liquid |
| الوصف: | Tools that provide absolute quantification of biomolecules, particularly of proteins and their post-translational modifications, without needing suitable specific standards, are urgently demanded nowadays. To this end, we have significantly improved the recently introduced strategy based on CH addition to the plasma for absolute quantification of biomolecules using HPLC-ICP-MS. Addition of CO has been optimized and finally selected as a safer, more efficient quantitative strategy that is able to provide constant ( The authors acknowledge financial support of Spanish Ministry of Economy and Competitiveness through CTQ2016-79412-P, and BES-2014-068032 and EEBB-I-17-12654 (F.C.C). |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6565ad832b5a77dffc39cf4acb62c54 http://hdl.handle.net/10651/51085 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e6565ad832b5a77dffc39cf4acb62c54 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |