Biomineralization of calcium phosphate revealed by in situ liquid-phase electron microscopy

التفاصيل البيبلوغرافية
العنوان: Biomineralization of calcium phosphate revealed by in situ liquid-phase electron microscopy
المؤلفون: Leyla Soleymani, Xiaoyue Wang, Jie Yang, Carmen M. Andrei, Kathryn Grandfield
المصدر: Communications Chemistry, Vol 1, Iss 1, Pp 1-7 (2018)
بيانات النشر: Nature Publishing Group, 2018.
سنة النشر: 2018
مصطلحات موضوعية: Simulated body fluid, Nucleation, chemistry.chemical_element, Crystal growth, General Chemistry, Calcium, Biochemistry, Mineralization (biology), law.invention, lcsh:Chemistry, chemistry, lcsh:QD1-999, Transmission electron microscopy, law, Materials Chemistry, Biophysics, Environmental Chemistry, Electron microscope, Biomineralization
الوصف: Calcium phosphate biomineralization is essential to the formation of bones and teeth, and other pathological calcifications. Unravelling the mechanism of calcium phosphate nucleation and growth contributes significantly to understanding diseases caused by pathological mineralization, and also to designing biomimetic materials with suitable properties. Recently, calcium phosphate was proposed to mineralize following a non-classical crystal growth pathway of pre-nucleation cluster aggregation. Liquid-phase transmission electron microscopy allows dynamic processes to be recorded continuously inside liquid. Here we present direct evidence, based on continuous monitoring in liquid, to confirm that calcium phosphate mineralization from simulated body fluid occurs by particle attachment, shown with nanoscale spatial resolution and sufficient temporal resolution. This work may lay the foundation for future investigation of mineralization in other relevant biological systems in humans and vertebrates. Calcium phosphate is an important biomineral and is thought to crystallise through non-classical pathways. Here, time-resolved liquid-phase transmission electron microscopy offers confirmatory evidence gained through continuous monitoring of mineralisation in solution.
اللغة: English
تدمد: 2399-3669
DOI: 10.1038/s42004-018-0081-4
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5bc7785b9a39b9fadb6c65c5082a3b0
http://link.springer.com/article/10.1038/s42004-018-0081-4
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....e5bc7785b9a39b9fadb6c65c5082a3b0
قاعدة البيانات: OpenAIRE
الوصف
تدمد:23993669
DOI:10.1038/s42004-018-0081-4