Burkholderia thailandensis: the Main Bacteria Biodegrading Fipronil in Fertilized Soil with Assessment by a QuEChERS/GC-MS Method
| العنوان: | Burkholderia thailandensis: the Main Bacteria Biodegrading Fipronil in Fertilized Soil with Assessment by a QuEChERS/GC-MS Method |
|---|---|
| المؤلفون: | Ana Carolina Urbaczek, Luciana Tereza Dias Cappelini, Eny Maria Vieira, Patrícia de Fátima Menegoci Eugênio, Eloisa Pozzi, Elma Neide Vasconcelos Martins Carrilho, Juliana Vieira Alberice, Emanuel Carrilho, Lia Gracy Rocha Diniz |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Detection limit, Chromatography, Burkholderia thailandensis, biology, Relative standard deviation, General Chemistry, 010501 environmental sciences, biology.organism_classification, Quechers, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Gas chromatography–mass spectrometry, CROMATOGRAFIA A GÁS, Fipronil, Bacteria, 0105 earth and related environmental sciences, Burkholderia sp |
| الوصف: | Due to the expansion of sugarcane crops and the high costs of agricultural inputs, an alternative for fertilization of the soil is the use of humic substances (HS) because they improve its physical-chemical properties. The objective of this study was to assess the biological degradation of fipronil in soil supplemented with HS. For determination of fipronil and its metabolites, we validated a quick, easy, cheap, effective, rugged, and safe gas chromatography-mass spectrometry (QuEChERS/GC-MS) method, which yielded detection and quantification limits of 15.0 and 62.5 ng g-1, respectively. The method presented linearity of 0.99, precision between 1.5 and 10.9%, while the recovery ranged from 78 to 98%, with precision (relative standard deviation (RSD)) < 5%. The strain B. thailandensis of Burkholderia sp. grew under typical conditions for degradation of fipronil. Eight treatments assessed the degradation by B. thailandensis in the presence of HS. The metabolites produced in the experiment, although detected, could not be quantified because they were below the limit of quantification of the method. Our results showed that B. thailandensis has the potential to degrade fipronil, and that HS is not toxic to the microbiota, and does not inactivate fipronil. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e57958959c9da2578b6b74c2d1fe3e39 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e57958959c9da2578b6b74c2d1fe3e39 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |