Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation
| العنوان: | Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation |
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| المؤلفون: | Norbert Stockhofe-Zurwieden, Huub F. J. Savelkoul, Artur Summerfield, Sandra Vreman, Gaël Auray, Annemarie Rebel |
| المصدر: | Developmental and Comparative Immunology, 84, 361-370 Vreman, Sandra; Auray, Gael; Savelkoul, Huub F J; Rebel, Annemarie; Summerfield, Artur; Stockhofe-Zurwieden, Norbert (2018). Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation. Developmental and Comparative Immunology, 84, pp. 361-370. Elsevier 10.1016/j.dci.2018.03.012 <http://dx.doi.org/10.1016/j.dci.2018.03.012> Developmental and Comparative Immunology 84 (2018) Developmental & Comparative Immunology |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Swine, Porcine, Cell Separation, Dierenwelzijn en gezondheid, 0302 clinical medicine, Neonate, Cells, Cultured, Innate immunity, Antigen Presentation, 630 Agriculture, Bacteriologie, Cell Differentiation, hemic and immune systems, Bacteriology, Host Pathogen Interaction & Diagnostics, Flow Cytometry, Interleukin-12, 3. Good health, Oligodeoxyribonucleotides, Tumor necrosis factor alpha, Dendritic cell, Lipoproteins, Immunology, chemical and pharmacologic phenomena, Celbiologie en Immunologie, Biology, Peripheral blood mononuclear cell, Toll like receptor ligand, 03 medical and health sciences, Antigens, CD, Animals, Animal Health & Welfare, Antigen-presenting cell, Host Pathogen Interaction & Diagnostics, Innate immune system, TLR9, Bacteriology, Dendritic Cells, Molecular biology, Host Pathogen Interactie & Diagnostiek, Toll-Like Receptor 2, TLR2, 030104 developmental biology, Animals, Newborn, Cell Biology and Immunology, Toll-Like Receptor 9, Bacteriologie, Host Pathogen Interactie & Diagnostiek, WIAS, 570 Life sciences, biology, CD80, 030215 immunology, Developmental Biology |
| الوصف: | The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 0145-305X |
| DOI: | 10.1016/j.dci.2018.03.012 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4172179f5c2fbf9a85bf0d74179b644 https://research.wur.nl/en/publications/neonatal-porcine-blood-derived-dendritic-cell-subsets-show-activa |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e4172179f5c2fbf9a85bf0d74179b644 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0145305X |
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| DOI: | 10.1016/j.dci.2018.03.012 |