A miR 169 isoform regulates specificNF ‐YA targets and root architecture inA rabidopsis
| العنوان: | A mi |
|---|---|
| المؤلفون: | Thomas Blein, Linnan Ma, Céline Sorin, Marie Declerck, Caroline Hartmann, Maria Fransiska Njo, Aurélie Christ, Christine Lelandais-Brière, Martin Crespi, Tom Beeckman |
| المساهمون: | Institute of Plant Sciences, Agricultural Research Organization-The Volcani Center, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences du Végétal, UPR 2355, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Department of Plant Systems Biology, State University of Ghent, Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Conseil General de l'Essonne, French Ministry of Education, ROOT KBBE project, LabEx SPS, Interuniversity Attraction Poles Programme from the Belgian Federal Science Policy Office [IAP VI/33, IUAP P7/29], Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB) |
| المصدر: | New Phytologist New Phytologist, Wiley, 2014, 202 (4), pp.1197-1211. ⟨10.1111/nph.12735⟩ |
| بيانات النشر: | Wiley, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Gene isoform, root branching, Physiology, Meristem, Arabidopsis, Gene Expression, Transcription factor complex, Plant Science, Biology, Plant Roots, Gene Expression Regulation, Plant, Genes, Reporter, Gene expression, RNA Isoforms, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene, Transcription factor, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Nuclear Factor Y subunit A (NF-YA), Messenger RNA, Arabidopsis Proteins, Plants, Genetically Modified, biology.organism_classification, Molecular biology, Cell biology, MicroRNAs, Phenotype, CCAAT-Binding Factor, RNA, Plant, miR169 isoforms, mimicry |
| الوصف: | International audience; In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis. |
| تدمد: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.12735 |
| DOI: | 10.1111/nph.12735⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e34923e08818157ca6ee022360ac355e https://doi.org/10.1111/nph.12735 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e34923e08818157ca6ee022360ac355e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
|---|---|
| DOI: | 10.1111/nph.12735 |