Untargeted serum metabolites profiling in high-fat diet mice supplemented with enhanced palm tocotrienol-rich fraction using UHPLC-MS
| العنوان: | Untargeted serum metabolites profiling in high-fat diet mice supplemented with enhanced palm tocotrienol-rich fraction using UHPLC-MS |
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| المؤلفون: | Jen Kit Tan, Musalmah Mazlan, Amir Hakimi Mohd Sakri, Mardiana Abdul Aziz, Effendi Ibrahim, Siti Hamimah Sheikh Abdul Kadir, Danial Efendy Goon, Sharaniza Ab-Rahim, Norizal Mohd Noor |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-19 (2021) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Science, Diseases, Arecaceae, Palm Oil, Diet, High-Fat, medicine.disease_cause, Biochemistry, Article, Mass Spectrometry, Mice, chemistry.chemical_compound, Non-alcoholic Fatty Liver Disease, Internal medicine, medicine, Animals, Metabolomics, Beta oxidation, Chromatography, High Pressure Liquid, Hypoxanthine, chemistry.chemical_classification, Multidisciplinary, Molecular medicine, Tocotrienols, Fatty liver, food and beverages, Xanthine, medicine.disease, Immunohistochemistry, Sphingolipid, Amino acid, Disease Models, Animal, Endocrinology, Liver, chemistry, Dietary Supplements, Metabolome, Medicine, Arachidonic acid, Biomarkers, Oxidative stress |
| الوصف: | Excessive high fat dietary intake promotes risk of developing non-alcoholic fatty liver disease (NAFLD) and predisposed with oxidative stress. Palm based tocotrienol-rich fraction (TRF) has been reported able to ameliorate oxidative stress but exhibited poor bioavailability. Thus, we investigated whether an enhanced formulation of TRF in combination with palm kernel oil (medium-chain triglycerides) (ETRF) could ameliorate the effect of high-fat diet (HFD) on leptin-deficient male mice. All the animals were divided into HFD only (HFD group), HFD supplemented with ETRF (ETRF group) and HFD supplemented with TRF (TRF group) and HFD supplemented with PKO (PKO group). After 6 weeks, sera were collected for untargeted metabolite profiling using UHPLC-Orbitrap MS. Univariate analysis unveiled alternation in metabolites for bile acids, amino acids, fatty acids, sphingolipids, and alkaloids. Bile acids, lysine, arachidonic acid, and sphingolipids were downregulated while xanthine and hypoxanthine were upregulated in TRF and ETRF group. The regulation of these metabolites suggests that ETRF may promote better fatty acid oxidation, reduce oxidative stress and pro-inflammatory metabolites and acts as anti-inflammatory in fatty liver compared to TRF. Metabolites regulated by ETRF also provide insight of its role in fatty liver. However, further investigation is warranted to identify the mechanisms involved. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e28830373bfaf9018d5c7fa2d481b3ff https://doaj.org/article/4dc9acc044244c3797c62d6ffff57e61 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e28830373bfaf9018d5c7fa2d481b3ff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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