PPR-B-31: a new maintainer allele in the male-fertility restorer gene of radish ([i]Raphanus sativus[/i]) Ogura cytoplasm
| العنوان: | PPR-B-31: a new maintainer allele in the male-fertility restorer gene of radish ([i]Raphanus sativus[/i]) Ogura cytoplasm |
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| المؤلفون: | Aleksandra Korzeniewska, Renata Słomnicka, Anna Hawliczek-Strulak, Régine Delourme, Katarzyna Niemirowicz-Szczytt, Iwona Bartkowiak-Broda, Grzegorz Bartoszewski |
| المساهمون: | Warsaw Agricultural University, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Plant Breeding and Acclimatization Institute, Polish Ministry of Agriculture and Rural Development project, AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| المصدر: | Plant Breeding Plant Breeding, Wiley, 2015, 134 (5), pp.557-563. ⟨10.1111/pbr.12303⟩ Plant Breeding, 2015, 134 (5), pp.557-563. ⟨10.1111/pbr.12303⟩ |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Genetics, Pseudogene, Software maintainer, Raphanus, Locus (genetics), Plant Science, PPR-B gene, Biology, biology.organism_classification, male sterility, Intergenic region, Cytoplasm, radish, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Allele, Rfo locus, Agronomy and Crop Science, Gene, fertility restoration |
| الوصف: | International audience; The PPR-B gene is responsible for male-fertility restoration of the Ogura-type male-sterile radish plants, and it is located in the complex Rfo locus in the vicinity of similar PPR-A gene and PPR-C pseudogene. The aim of this study was to identify PPR-B alleles and understand the structure of the Rfo locus in radish breeding lines. Five lines of radish with normal male-fertile cytoplasm were tested. The entire PPR-B gene was amplified, sequenced and allelic PPR-B sequences were identified. The results indicated that the maintainer lines 7, 15 and 21 contained a non-restoring form of PPR-B protein. A unique PPR-B was found in lines 24/15 and 31 that are restorer and maintainer lines, respectively. The substitutions might be responsible for the loss of a restoring function of the PPR-B-31 allele. Amplification of the PPR-A/PPR-B and PPR-B/PPR-C intergenic regions allowed to identify rearrangements within Rfo locus. Obtained results confirm the wide allelic variation within the Rfo locus, as well as high genetic complexity of the fertility restoration mechanism in radish |
| اللغة: | English |
| تدمد: | 0179-9541 1439-0523 |
| DOI: | 10.1111/pbr.12303⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e28061a59fb5441e30c3df8cef892e7d https://hal.archives-ouvertes.fr/hal-01252656 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e28061a59fb5441e30c3df8cef892e7d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01799541 14390523 |
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| DOI: | 10.1111/pbr.12303⟩ |