Cloning of an Interleukin-3 Receptor Gene: a Member of a Distinct Receptor Gene Family
| العنوان: | Cloning of an Interleukin-3 Receptor Gene: a Member of a Distinct Receptor Gene Family |
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| المؤلفون: | Shin Yonehara, Jolanda Schreurs, Ai Ishii, Atsushi Miyajima, Kazuo Maruyama, Daniel M. Gorman, Naoto Itoh, Ichiro Yahara, Ken-ichi Arai |
| المصدر: | Science. 247:324-327 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Molecular Sequence Data, Interleukin-17 receptor, Biology, Transfection, Tropomyosin receptor kinase C, Mice, Sequence Homology, Nucleic Acid, Escherichia coli, Animals, 5-HT5A receptor, Amino Acid Sequence, Cloning, Molecular, Receptors, Immunologic, Nuclear receptor co-repressor 1, Common gamma chain, Multidisciplinary, Nucleic Acid Hybridization, DNA, Fibroblasts, Protein-Tyrosine Kinases, Molecular biology, Receptors, Interleukin-3, Interleukin-21 receptor, ROR1, Interleukin-3, Estrogen-related receptor gamma, DNA Probes, Plasmids |
| الوصف: | Interleukin-3 (IL-3) binds to its receptor with high and low affinities, induces tyrosine phosphorylation, and promotes the proliferation and differentiation of hematopoietic cells. A binding component of the IL-3 receptor was cloned. Fibroblasts transfected with the complementary DNA bound IL-3 with a low affinity [dissociation constant (Kd) of 17.9 +/- 3.6 nM]. No consensus sequence for a tyrosine kinase was present in the cytoplasmic domain. Thus, additional components are required for a functional high affinity IL-3 receptor. A sequence comparison of the IL-3 receptor with other cytokine receptors (erythropoietin, IL-4, IL-6, and the beta chain IL-2 receptor) revealed a common motif of a distinct receptor gene family. |
| تدمد: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.2404337 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e20028d06bf264dd75f9e32988916f01 https://doi.org/10.1126/science.2404337 |
| رقم الانضمام: | edsair.doi.dedup.....e20028d06bf264dd75f9e32988916f01 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959203 00368075 |
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| DOI: | 10.1126/science.2404337 |