Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells
| العنوان: | Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells |
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| المؤلفون: | Bin Zheng, Jie Zhuang, Hong Sun, Chenxia Xu, Guifeng Zhang, Mumu Xie, Jiaqin Cai, Xuhui Huang |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Curcumin, Epithelial-Mesenchymal Transition, Cell Survival, Cell, Breast Neoplasms, Biochemistry, Metastasis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, breast cancer, tamoxifen-resistance, Cell Movement, Pancreatic cancer, Genetics, medicine, Humans, Epithelial–mesenchymal transition, Molecular Biology, Cell Proliferation, Oncogene, Dose-Response Relationship, Drug, business.industry, Cancer, Drug Synergism, Articles, Cell cycle, medicine.disease, H19 imprinted maternally expressed transcript, female genital diseases and pregnancy complications, Gene Expression Regulation, Neoplastic, Tamoxifen, 030104 developmental biology, medicine.anatomical_structure, Oncology, chemistry, Drug Resistance, Neoplasm, 030220 oncology & carcinogenesis, embryonic structures, Cancer research, MCF-7 Cells, Molecular Medicine, Female, RNA, Long Noncoding, business |
| الوصف: | The H19 long non‑coding RNA is involved in the development of tamoxifen resistance in breast cancer. However, the relationship between H19 and the metastatic potential and treatment options for tamoxifen‑resistant (TAMR) breast cancer is not completely understood. Curcumin inhibits cellular proliferation, migration and invasiveness in several cancer types, including pancreatic cancer, breast cancer and chronic myeloid leukemia. The present study aimed to investigate the role of H19 in MCF‑7/TAMR cell epithelial‑mesenchymal transition (EMT), migration and invasiveness, and to assess the ability of curcumin to inhibit H19‑mediated effects. Reverse transcription‑quantitative PCR and western blot analysis were conducted to detect the gene or protein expression. Cell Counting Kit‑8, wound healing and Transwell invasion assays were performed to estimate the capabilities of cell viability, invasion and migration. H19 overexpression enhanced MCF‑7/TAMR cell EMT, invasion and migration by upregulating Snail. Furthermore, curcumin notably decreased the expression levels of epithelial marker E‑cadherin and markedly increased the expression levels of mesenchymal marker N‑cadherin in MCF‑7/TAMR cells compared with the control group. In addition, following treatment with curcumin for 48 h, H19 expression was decreased in a dose‑dependent manner. Moreover, curcumin treatment for 48 h significantly attenuated H19‑induced alterations in N‑cadherin and E‑cadherin expression levels. Curcumin also prevented H19‑induced invasion and migration. The present study indicated that H19 may serve as a promoting factor of EMT, invasion and migration in MCF‑7/TAMR cells, suggesting that curcumin may prevent H19‑associated metastasis. Therefore, curcumin may serve as a promising therapeutic drug for patients with TAMR breast cancer. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0a5e0b254f29edf3759786785df4efb http://europepmc.org/articles/PMC7673326 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e0a5e0b254f29edf3759786785df4efb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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