Processive cleavage of concatemer DNA duplexes by Eco RII restriction endonuclease
| العنوان: | Processive cleavage of concatemer DNA duplexes by Eco RII restriction endonuclease |
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| المؤلفون: | Gromova Es, Shabarova Za, A.A. Yolov |
| المصدر: | Molecular Biology Reports. 10:173-176 |
| بيانات النشر: | Springer Science and Business Media LLC, 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | chemistry.chemical_classification, biology, Base pair, Concatemer, Stereochemistry, Nucleic Acid Heteroduplexes, DNA, DNA Restriction Enzymes, General Medicine, In Vitro Techniques, Cleavage (embryo), Substrate Specificity, Endonuclease, chemistry.chemical_compound, Restriction enzyme, Enzyme, chemistry, Biochemistry, Cleave, Genetics, biology.protein, Deoxyribonucleases, Type II Site-Specific, Molecular Biology |
| الوصف: | Eco RII restriction endonuclease cleaves synthetic DNA-duplexes in which the recognition sites of this enzyme (5'...CCATGG...) are repeated every 9 base pairs with the alternating orientation of the central AT pair. It operates in a processive mode, i.e. the bound enzyme molecule slides along the substrate toward neighboring recognition sites. Nona-nucleotides are the main products of the cleavage. The data obtained neighboring recognition sites. Nona-nucleotides are the main products of the cleavage. The data obtained point to the capability of Eco RII endonuclease to recognize and cleave the substrate under both possible orientations of the central AT-pair of the recognition site with respect to the bound enzyme molecule. These data also show the close similarity of DNA structures in a complex with the enzyme and without. |
| تدمد: | 1573-4978 0301-4851 |
| DOI: | 10.1007/bf00778525 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e02424a3142ce274828ee02fdec7fb11 https://doi.org/10.1007/bf00778525 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e02424a3142ce274828ee02fdec7fb11 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734978 03014851 |
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| DOI: | 10.1007/bf00778525 |