Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages
| العنوان: | Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages |
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| المؤلفون: | María Salomé Sirerol-Piquer, Allen D. Seol, Andrew Kodani, Jeremy F. Reiter, José Manuel García-Verdugo |
| المصدر: | The EMBO journal, vol 32, iss 4 EMBO JOURNAL r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) instname |
| بيانات النشر: | eScholarship, University of California, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Centriole, Knockout, Kinesins, Biology, centriole cohesion, Kif3a, Medical and Health Sciences, Article, General Biochemistry, Genetics and Molecular Biology, Mice, Microtubule, Intraflagellar transport, Information and Computing Sciences, Animals, Humans, KIF3A, Microtubule anchoring, Molecular Biology, Centrioles, Mice, Knockout, General Immunology and Microbiology, General Neuroscience, Cilium, Tumor Suppressor Proteins, Nuclear Proteins, Kinesin, Dynactin Complex, Biological Sciences, Cell biology, Cytoskeletal Proteins, centrosome, Centrosome, Hela Cells, Dynactin, Generic health relevance, Microtubule-Associated Proteins, p150(Glued), HeLa Cells, subdistal appendage, Developmental Biology |
| الوصف: | Formation of cilia, microtubule-based structures that function in propulsion and sensation, requires Kif3a, a subunit of Kinesin II essential for intraflagellar transport (IFT). We have found that, Kif3a is also required to organize centrioles. In the absence of Kif3a, the subdistal appendages of centrioles are disorganized and lack p150(Glued) and Ninein. Consequently, microtubule anchoring, centriole cohesion and basal foot formation are abrogated by loss of Kif3a. Kif3a localizes to the mother centriole and interacts with the Dynactin subunit p150(Glued) . Depletion of p150(Glued) phenocopies the effects of loss of Kif3a, indicating that Kif3a recruitment of p150(Glued) is critical for subdistal appendage formation. The transport functions of Kif3a are dispensable for subdistal appendage organization as mutant forms of Kif3a lacking motor activity or the motor domain can restore p150(Glued) localization. Comparison to cells lacking Ift88 reveals that the centriolar functions of Kif3a are independent of IFT. Thus, in addition to its ciliogenic roles, Kif3a recruits p150(Glued) to the subdistal appendages of mother centrioles, critical for centrosomes to function as microtubule-organizing centres. The EMBO Journal (2013) 32, 597-607. doi: 10.1038/emboj.2013.3; Published online 5 February 2013 |
| وصف الملف: | application/pdf |
| تدمد: | 0261-4189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e01d04f5c96b01d13533180e4ef216a5 https://escholarship.org/uc/item/0pv3q3jp |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e01d04f5c96b01d13533180e4ef216a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02614189 |
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