Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death
| العنوان: | Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
|---|---|
| المؤلفون: | Romildo Romualdo Weiss, Luiz Ernandes Kozicki, Tácia Gomes Bergstein-Galan, Sony Dimas Bicudo |
| المساهمون: | Federal University of Paraná, Pontifical Catholic University of Paraná, Universidade Estadual Paulista (Unesp) |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, endocrine system, Sperm Retrieval, Pregnancy Rate, medicine.medical_treatment, Semen, Fertilization in Vitro, Biology, Insemination, law.invention, Andrology, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Food Animals, law, Pregnancy, Blood plasma, medicine, Animals, Ejaculation, reproductive and urinary physiology, Insemination, Artificial, Seminal plasma, Cryopreservation, Epididymis, 030219 obstetrics & reproductive medicine, Sheep, urogenital system, Artificial insemination, Extender, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, Sperm, Spermatozoa, Motion pattern, Death, Semen Analysis, Ram, medicine.anatomical_structure, Vagina, Animal Science and Zoology, Female, Semen Preservation |
| الوصف: | Made available in DSpace on 2018-12-11T17:15:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P < 0.05) percentage of SP2 and lower (P |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0104129e43c01353aa540e18a8d103b |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e0104129e43c01353aa540e18a8d103b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |