New Far‐Red and Near‐Infrared Fluorescent Phycobiliproteins with Excellent Brightness and Photostability
| العنوان: | New Far‐Red and Near‐Infrared Fluorescent Phycobiliproteins with Excellent Brightness and Photostability |
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| المؤلفون: | Ya‐Nan Hou, Wen‐Long Ding, Xiang‐Xiang Jiang, Ji‐Ling Hu, Zi‐Zhu Tan, Kai‐Hong Zhao |
| المصدر: | ChemBioChem. 23 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mammals, Bacterial Proteins, Microscopy, Fluorescence, Biliverdine, Organic Chemistry, Animals, Molecular Medicine, Phycobiliproteins, Molecular Biology, Biochemistry, Biomarkers, Fluorescent Dyes |
| الوصف: | Far-red and near-infrared fluorescent proteins can be used as fluorescence biomarkers in the region of maximal transmission of most tissues and facilitate multiplexing. Recently, we reported the generation and properties of far-red and near-infrared fluorescent phycobiliproteins, termed BeiDou Fluorescent Proteins (BDFPs), which can covalently bind the more readily accessible biliverdin. Far-red BDFPs maximally fluoresce at ∼670 nm, while near-infrared BDFPs fluoresce at ∼710 nm. In this work, we molecularly evolved BDFPs as follows: (a) mutations L58Q, S68R and M81K of BDFPs, which can maximally enhance the effective brightness in vivo by 350 %; (b) minimization and monomerization of far-red BDFPs 2.1, 2.2, 2.3, and near-infrared BDFPs 2.4, 2.5 and 2.6. These newly developed BDFPs are remarkably brighter than the formerly reported far-red and near-infrared fluorescent proteins. Their advantages are demonstrated by biolabeling in mammalian cells using super-resolution microscopy. |
| تدمد: | 1439-7633 1439-4227 |
| DOI: | 10.1002/cbic.202200267 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfe0c250751e6d38875ef5259ac1e87a https://doi.org/10.1002/cbic.202200267 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....dfe0c250751e6d38875ef5259ac1e87a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14397633 14394227 |
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| DOI: | 10.1002/cbic.202200267 |