Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro
| العنوان: | Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro |
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| المؤلفون: | Jamal Tazi, Hélène Liénard, Henrik Leffers, Jérôme Lacoste, Jean-Louis Mergny, Claude Hélène, Emmanuel Labourier, Laurent Lacroix, Mojgan Djavaheri-Mergny |
| المساهمون: | Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | CIÊNCIAVITAE Scopus-Elsevier ResearcherID Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2000, 28 (7), pp.1564--1575. ⟨10.1093/nar/28.7.1564⟩ |
| مصطلحات موضوعية: | RNA-binding protein, DNA-binding protein sf2/asf splicing factor g-quartet formation saccharomyces-cerevisiae in-vitro yeast telomeres c-myc ribonucleoprotein-k circular-dichroism trypanosoma-brucei, Biology, 010402 general chemistry, Heterogeneous ribonucleoprotein particle, 01 natural sciences, Article, Heterogeneous-Nuclear Ribonucleoproteins, Heterogeneous-Nuclear Ribonucleoprotein K, 03 medical and health sciences, chemistry.chemical_compound, Cytosine, Genetics, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030304 developmental biology, Ribonucleoprotein, 0303 health sciences, Base Composition, Binding Sites, Base Sequence, Serine-Arginine Splicing Factors, Oligonucleotide, Nuclear Proteins, RNA-Binding Proteins, DNA, Telomere, Molecular biology, 0104 chemical sciences, Molecular Weight, chemistry, Ribonucleoproteins, Coding strand, HeLa Cells |
| الوصف: | Most studies on the structure of DNA in telomeres have been dedicated to the double-stranded region or the guanosine-rich strand and consequently little is known about the factors that may bind to the telomere cytosine-rich (C-rich) strand. This led us to investigate whether proteins exist that can recognise C-rich sequences. We have isolated several nuclear factors from human cell extracts that specifically bind the C-rich strand of vertebrate telomeres [namely a d(CCCTAA)(n) repeat] with high affinity and bind double-stranded telomeric DNA with a 100x reduced affinity. A biochemical assay allowed us to characterise four proteins of apparent molecular weights 66-64, 45 and 35 kDa, respectively. To identify these polypeptides we screened a lambda gt11-based cDNA expression library, obtained from human HeLa cells using a radiolabelled telomeric oligonucleotide as a probe. Two clones were purified and sequenced: the first corresponded to the hnRNP K protein and the second to the ASF/SF2 splicing factor. Confirmation of the screening results was obtained with recombinant proteins, both of which bind to the human telomeric C-rich strand in vitro. |
| تدمد: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/28.7.1564⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfdcc12e4cf973b0e34a7b509753fefa http://www.scopus.com/inward/record.url?eid=2-s2.0-0343384080&partnerID=MN8TOARS |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dfdcc12e4cf973b0e34a7b509753fefa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03051048 13624962 |
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| DOI: | 10.1093/nar/28.7.1564⟩ |