Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants
| العنوان: | Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants |
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| المؤلفون: | Margarita Pliner, Alejandro Sarrion-Perdigones, Asaph Aharoni, Antonio Granell, Efrat Weithorn, Noam Grossman, Dario Breitel, Ilana Rogachev, Diego Orzaez, Guy Polturak |
| المصدر: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Pigments, 0106 biological sciences, 0301 basic medicine, Plant metabolic engineering, Physiology, Nicotiana benthamiana, Plant Science, 01 natural sciences, chemistry.chemical_compound, Cytochrome P-450 Enzyme System, Gene Expression Regulation, Plant, Phylogeny, Genetics, Caryophyllales, biology, Pigmentation, food and beverages, Plants, Genetically Modified, Recombinant Proteins, Betaxanthins, Dihydroxyphenylalanine, Mirabilis jalapa, Phenotype, Biochemistry, Biosynthetic process, Beta vulgaris, Genetic Engineering, Betalains, Saccharomyces cerevisiae, Genes, Plant, 03 medical and health sciences, Betalain, Tobacco, BIOQUIMICA Y BIOLOGIA MOLECULAR, Betacyanins, Gene Silencing, Secondary metabolism, Gene Expression Profiling, MICROBIOLOGIA, biology.organism_classification, Biosynthetic Pathways, 030104 developmental biology, chemistry, Heterologous expression, Mirabilis, Gene function, 010606 plant biology & botany |
| الوصف: | [EN] Betalains are tyrosine-derived red-violet and yellow pigments, found in plants only of the Caryophyllales order. Although much progress has been made in recent years in the understanding of the betalain biosynthetic process, many questions remain open with regards to several of the proposed steps in the pathway. Most conspicuous by its absence is the characterization of the first committed step in the pathway, namely the 3-hydroxylation of tyrosine to form L-3,4-dihydroxyphenylalanine (L-DOPA). We used transcriptome analysis of the betalain-producing plants red beet (Beta vulgaris) and four o'clocks (Mirabilis jalapa) to identify a novel, betalain-related cytochrome P450-type gene, CYP76AD6, and carried out gene silencing and recombinant expression assays in Nicotiana benthamiana and yeast cells to examine its functionality. L-DOPA formation in red beet was found to be redundantly catalyzed by CYP76AD6 together with a known betalain-related enzyme, CYP76AD1, which was previously thought to only catalyze a succeeding step in the pathway. While CYP76AD1 catalyzes both L-DOPA formation and its subsequent conversion to cyclo-DOPA, CYP76AD6 uniquely exhibits only tyrosine hydroxylase activity. The new findings enabled us to metabolically engineer entirely red-pigmented tobacco plants through heterologous expression of three genes taking part in the fully decoded betalain biosynthetic pathway. |
| وصف الملف: | application/pdf |
| تدمد: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.13796 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df4738bf5a146d20d7486ffa6cf537c8 https://doi.org/10.1111/nph.13796 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....df4738bf5a146d20d7486ffa6cf537c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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| DOI: | 10.1111/nph.13796 |