Differentiation of sheeppox and goatpox viruses by polymerase Chain reaction-restriction fragment length polymorphism
| العنوان: | Differentiation of sheeppox and goatpox viruses by polymerase Chain reaction-restriction fragment length polymorphism |
|---|---|
| المؤلفون: | Gnanavel Venkatesan, Veerakyathappa Bhanuprakash, R. Yogisharadhya, Vinayagamurthy Balamurugan, Amit Kumar |
| المصدر: | Virol Sin |
| بيانات النشر: | Elsevier BV, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Sequence analysis, Molecular Sequence Data, Immunology, India, medicine.disease_cause, Polymerase Chain Reaction, Capripoxvirus, Viral Proteins, Virology, Sheeppox virus, medicine, Animals, Cluster Analysis, Point Mutation, Phylogeny, Sheeppox, Genetics, Polymorphism, Genetic, Sheep, biology, Goats, Goatpox virus, Sequence Analysis, DNA, biology.organism_classification, Restriction site, Restriction enzyme, DNA, Viral, Molecular Medicine, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length, Research Article |
| الوصف: | In the present study, the partial gene sequences of P32 protein, an immunogenic envelope protein of Capripoxviruses (CaPV), were analyzed to assess the genetic relationship among sheeppox and goatpox virus isolates, and restriction enzyme specific PCR-RFLP was developed to differentiate CaPV strains. A total of six goatpox virus (GTPV) and nine sheeppox virus (SPPV) isolates of Indian origin were included in the sequence analysis of the attachment gene. The sequence analysis revealed a high degree of sequence identity among all the Indian SPPV and GTPV isolates at both nucleotide and amino acid levels. Phylogenetic analysis showed three distinct clusters of SPPV, GTPV and Lumpy skin disease virus (LSDV) isolates. Further, multiple sequence alignment revealed a unique change at G120A in all GTPV isolates resulting in the formation of Dra I restriction site in lieu of EcoR I, which is present in SPPV isolates studied. This change was unique and exploited to develop restriction enzyme specific PCR-RFLP for detection and differentiation of SPPV and GTPV strains. The optimized PCR-RFLP was validated using a total of fourteen (n=14) cell culture isolates and twenty two (n=22) known clinical samples of CaPV. The Restriction Enzyme specific PCR-RFLP to differentiate both species will allow a rapid differential diagnosis during CaPV outbreaks particularly in mixed flocks of sheep and goats and could be an adjunct/supportive tool for complete gene or virus genome sequencing methods. |
| تدمد: | 1995-820X 1674-0769 |
| DOI: | 10.1007/s12250-012-3277-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df2a1dbceac61b8144da8f0955682db3 https://doi.org/10.1007/s12250-012-3277-2 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....df2a1dbceac61b8144da8f0955682db3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1995820X 16740769 |
|---|---|
| DOI: | 10.1007/s12250-012-3277-2 |