Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection
| العنوان: | Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection |
|---|---|
| المؤلفون: | Bryce E. Nickels, Yuanchao Zhang, Yu Zhang, Richard H. Ebright, Irina O. Vvedenskaya, Jared T. Winkelman, Richard L. Gourse, Jeremy G. Bird, Deanne Taylor |
| المصدر: | Science. 351:1090-1093 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, genetic structures, genetic processes, RNA polymerase II, Crystallography, X-Ray, Article, 03 medical and health sciences, chemistry.chemical_compound, Transcription (biology), RNA polymerase, RNA, Messenger, Promoter Regions, Genetic, RNA polymerase II holoenzyme, Transcription Initiation, Genetic, Gene Library, Transcription bubble, Genetics, Multidisciplinary, Bacteria, 030102 biochemistry & molecular biology, General transcription factor, biology, Promoter, DNA, bacterial infections and mycoses, Cell biology, enzymes and coenzymes (carbohydrates), 030104 developmental biology, chemistry, biology.protein, bacteria, Nucleic Acid Conformation, Transcription factor II F, Transcription Initiation Site |
| الوصف: | Choosing where to start transcription The RNA polymerase enzyme complex binds to the promoter of a gene and separates the two DNA strands. The subsequently formed “transcription bubble” is required for RNA synthesis to begin. How RNA polymerase chooses the exact DNA base at which it will start transcription has been unclear. Winkelman et al. show that a control element upstream of the start site is involved in helping RNA polymerase make this choice in bacteria. Start site selection involves promoter scrunching, where a stationary RNA polymerase unwinds and pulls DNA through the active site, scrunching the DNA of the transcription bubble. Science , this issue p. 1090 |
| تدمد: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.aad6881 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::deb1ec7bd4c316dafd15f0dcbcd27dd4 https://doi.org/10.1126/science.aad6881 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....deb1ec7bd4c316dafd15f0dcbcd27dd4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959203 00368075 |
|---|---|
| DOI: | 10.1126/science.aad6881 |