Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization
| العنوان: | Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization |
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| المؤلفون: | Katia Ortiz, Luis D. Patiño-Lopez, José A. Jiménez-Torres, Jose A. Gavira, Andry Cera-Manjarres, Janet Mendez, Madeline Torres-Lugo |
| المصدر: | Crystal Growth & Design |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Materials science, Nanotechnology, 02 engineering and technology, macromolecular substances, 010402 general chemistry, complex mixtures, 01 natural sciences, Article, law.invention, chemistry.chemical_compound, law, PEG ratio, General Materials Science, Crystallization, Supersaturation, technology, industry, and agriculture, General Chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Chemical engineering, chemistry, Self-healing hydrogels, Agarose, Lysozyme, 0210 nano-technology, Protein crystallization, Ethylene glycol |
| الوصف: | Poly(ethylene glycol) (PEG) hydrogels are highly biocompatible materials extensively used for biomedical and pharmaceutical applications, controlled drug release, and tissue engineering. In this work, PEG cross-linked hydrogels, synthesized under various conditions, were used to grow lysozyme crystals by the counterdiffusion technique. Crystallization experiments were conducted using a three-layer arrangement. Results demonstrated that PEG fibers were incorporated within lysozyme crystals controlling the final crystal shape. PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose. PEG hydrogels can also be used at higher concentrations (20–50% w/w) as a separation chamber (plug) in counterdiffusion experiments. In this case, PEG hydrogels control the diffusion of the crystallization agent and therefore may be used to tailor the supersaturation to fine-tune crystal size. As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel. The resulting crystals were of an approximate size of 500 μm. Lysozyme crystal grown in 10% PEG hydrogel showing a spherical shape. |
| تدمد: | 1528-7483 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de73f3000d19f374694c552f96a5a20b https://pubmed.ncbi.nlm.nih.gov/25383049 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....de73f3000d19f374694c552f96a5a20b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15287483 |
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