Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens
| العنوان: | Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens |
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| المؤلفون: | Harry Pink, Adam Talbot, Abi Graceson, Juliane Graham, Gill Higgins, Andrew Taylor, Alison C. Jackson, Maria Truco, Richard Michelmore, Chenyi Yao, Frances Gawthrop, David Pink, Paul Hand, John P. Clarkson, Katherine Denby |
| المصدر: | TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, vol 135, iss 7 |
| بيانات النشر: | eScholarship, University of California, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Technology, Agricultural and Veterinary Sciences, QH, Gene Expression Profiling, Quantitative Trait Loci, Plant Biology & Botany, General Medicine, Lettuce, Biological Sciences, Plant Leaves, Genetics, 2.1 Biological and endogenous factors, Aetiology, Infection, SB, Agronomy and Crop Science, Biotechnology |
| الوصف: | Key message We demonstrate genetic variation for quantitative resistance against important fungal pathogens in lettuce and its wild relatives, map loci conferring resistance and predict key molecular mechanisms using transcriptome profiling. Abstract Lactuca sativa L. (lettuce) is an important leafy vegetable crop grown and consumed globally. Chemicals are routinely used to control major pathogens, including the causal agents of grey mould (Botrytis cinerea) and lettuce drop (Sclerotinia sclerotiorum). With increasing prevalence of pathogen resistance to fungicides and environmental concerns, there is an urgent need to identify sources of genetic resistance to B. cinerea and S. sclerotiorum in lettuce. We demonstrated genetic variation for quantitative resistance to B. cinerea and S. sclerotiorum in a set of 97 diverse lettuce and wild relative accessions, and between the parents of lettuce mapping populations. Transcriptome profiling across multiple lettuce accessions enabled us to identify genes with expression correlated with resistance, predicting the importance of post-transcriptional gene regulation in the lettuce defence response. We identified five genetic loci influencing quantitative resistance in a F6 mapping population derived from a Lactuca serriola (wild relative) × lettuce cross, which each explained 5–10% of the variation. Differential gene expression analysis between the parent lines, and integration of data on correlation of gene expression and resistance in the diversity set, highlighted potential causal genes underlying the quantitative trait loci. |
| وصف الملف: | application/pdf |
| تدمد: | 0040-5752 1432-2242 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de34668aa1af8ab6f4fcf29e490bacad https://escholarship.org/uc/item/2ck008dm |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....de34668aa1af8ab6f4fcf29e490bacad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00405752 14322242 |
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