Isolation of a New Phlorotannin, a Potent Inhibitor of Carbohydrate-Hydrolyzing Enzymes, from the Brown Alga Sargassum patens
| العنوان: | Isolation of a New Phlorotannin, a Potent Inhibitor of Carbohydrate-Hydrolyzing Enzymes, from the Brown Alga Sargassum patens |
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| المؤلفون: | Yasuko Kawamura-Konishi, Toshiyuki Sakaki, Noriyuki Nakajima, Miki Saito, Toshiki Enomoto, Natsuko Watanabe, Takane Katayama |
| المصدر: | Journal of Agricultural and Food Chemistry. 60:5565-5570 |
| بيانات النشر: | American Chemical Society (ACS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Phloroglucinol, Quercetagetin, Pancreatic alpha-Amylases, Biology, Phaeophyta, Phlorotannin, chemistry.chemical_compound, Japan, medicine, Animals, Humans, Enzyme Inhibitors, Acarbose, chemistry.chemical_classification, Sargassum, General Chemistry, biology.organism_classification, Enzyme assay, Rats, Kinetics, Enzyme, chemistry, Biochemistry, Salivary alpha-Amylases, Enzyme inhibitor, biology.protein, General Agricultural and Biological Sciences, medicine.drug |
| الوصف: | Ethanol extracts from 15 kinds of marine algae collected from the coast of the Noto Peninsula in Japan were examined for their inhibitory effects on human salivary α-amylase. Four extracts significantly suppressed the enzyme activity. An inhibitor was purified from the extract of Sargassum patens . The compound was a new phloroglucinol derivative, 2-(4-(3,5-dihydroxyphenoxy)-3,5-dihydroxyphenoxy) benzene-1,3,5-triol (DDBT), which strongly suppressed the hydrolysis of amylopectin by human salivary and pancreatic α-amylases. The 50% inhibitory activity (IC(50)) for α-amylase inhibition of DDBT (3.2 μg/mL) was much lower than that of commercially available α-amylase inhibitors, acarbose (26.3 μg/mL), quercetagetin (764 μg/mL), and α-amylase inhibitor from Triticum aestivum (88.3 μg/mL). A kinetic study indicated that DDBT was a competitive α-amylase inhibitor with a K(i) of 1.8 μg/mL. DDBT also inhibited rat intestinal α-glucosidase with an IC(50) value of 25.4 μg/mL for sucrase activity and 114 μg/mL for maltase activity. These results suggest that DDBT, a potent inhibitor of carbohydrate-hydrolyzing enzymes, may be useful as a natural nutraceutical to prevent diabetes. |
| تدمد: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf300165j |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde729f096f67930057b87cde84ba44a https://doi.org/10.1021/jf300165j |
| رقم الانضمام: | edsair.doi.dedup.....dde729f096f67930057b87cde84ba44a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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| DOI: | 10.1021/jf300165j |