Intravital microscopic optical coherence tomography imaging to assess mucus-mobilizing interventions for muco-obstructive lung disease in mice
| العنوان: | Intravital microscopic optical coherence tomography imaging to assess mucus-mobilizing interventions for muco-obstructive lung disease in mice |
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| المؤلفون: | Gereon Hüttmann, Hinnerk Schulz-Hildebrandt, Peter König, Marcus A. Mall, Mario Pieper |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology |
| بيانات النشر: | American Physiological Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Intravital Microscopy, muco-obstructive disease, Physiology, medicine.medical_treatment, Mice, Transgenic, Cystic fibrosis, Mice, 03 medical and health sciences, 0302 clinical medicine, In vivo, Physiology (medical), medicine, Animals, Humans, Lung Diseases, Obstructive, Epithelial Sodium Channels, Saline, mucus-mobilizing therapy, COPD, optical coherence tomography, Lung, business.industry, Cell Biology, respiratory system, medicine.disease, Mucus, Obstructive lung disease, Hypertonic saline, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, 030228 respiratory system, Mucociliary Clearance, Innovative Methodology, intravital imaging, business, Tomography, Optical Coherence |
| الوصف: | Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma, and COPD, and the development of more effective mucus-mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined whether newly developed high-speed microscopic optical coherence tomography (mOCT) is sensitive to detect and compare in vivo effects of inhaled isotonic saline, hypertonic saline, and bicarbonate on mucus mobilization and clearance in Scnn1b-transgenic mice with muco-obstructive lung disease. In vivo mOCT imaging showed that inhaled isotonic saline-induced rapid mobilization of mucus that was mainly transported as chunks from the lower airways of Scnn1b-transgenic mice. Hypertonic saline mobilized a significantly greater amount of mucus that showed a more uniform distribution compared with isotonic saline. The addition of bicarbonate-to-isotonic saline had no effect on mucus mobilization, but also led to a more uniform mucus layer compared with treatment with isotonic saline alone. mOCT can detect differences in response to mucus-mobilizing interventions in vivo, and may thus support the development of more effective therapies for patients with muco-obstructive lung diseases. |
| تدمد: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00287.2019 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd4ce7a90a407e5cb1c8ef35c26ba6ee https://doi.org/10.1152/ajplung.00287.2019 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dd4ce7a90a407e5cb1c8ef35c26ba6ee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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| DOI: | 10.1152/ajplung.00287.2019 |