Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?
| العنوان: | Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives? |
|---|---|
| المؤلفون: | Teresa Tomás-Chisbert, Lucía Murgui-Bosch, Alessandra Bordoni, Veronica Valli, Francesca Danesi, Mattia Di Nunzio, Lidia Tomás-Cobos |
| المساهمون: | Di Nunzio, Mattia, Valli, Veronica, Tomás-Cobos, Lidia, Tomás-Chisbert, Teresa, Murgui-Bosch, Lucía, Danesi, Francesca, Bordoni, Alessandra |
| المصدر: | BMC Complementary and Alternative Medicine BMC Complementary and Alternative Medicine, Vol 17, Iss 1, Pp 1-14 (2017) |
| بيانات النشر: | Zenodo, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Biomedical Research, Docosahexaenoic Acids, Cell Survival, Cytotoxicity, Protocatechuic acid, Pharmacology, Models, Biological, Anthocyanins, Biological Factors, 03 medical and health sciences, chemistry.chemical_compound, Glucosides, In vivo, Toxicity Tests, Hydroxybenzoates, Propionate, Humans, Medicine, HepG2 cells, Cyanidin-3-O-glucoside, business.industry, Biological activity, lcsh:Other systems of medicine, Hep G2 Cells, General Medicine, lcsh:RZ201-999, In vitro, 3. Good health, Docosahexaenoic acid, 030104 developmental biology, Complementary and alternative medicine, Biochemistry, chemistry, Hepg2 cells, Toxicity, Propionates, business, Research Article |
| الوصف: | Background: Foodstuffs of both plant and animal origin contain a wide range of bioactive compounds. Although human intervention studies are mandatory to assess the health effects of bioactives, the in vitro approach is often used to select the most promising molecules to be studied in vivo. To avoid misleading results, concentration and chemical form, exposure time, and potential cytotoxicity of the tested bioactives should be carefully set prior to any other experiments.Methods: In this study the possible cytotoxicity of different bioactives (docosahexaenoic acid, propionate, cyanidin-3-O-glucoside, protocatechuic acid), was investigated in HepG2 cells using different methods. Bioactives were supplemented to cells at different concentrations within the physiological range in human blood, alone or in combination, considering two different exposure times.Results: Reported data clearly evidence that in vitro cytotoxicity is tightly related to the exposure time, and it varies among bioactives, which could exert a cytotoxic effect even at a concentration within the in vivo physiological blood concentration range. Furthermore, co-supplementation of different bioactives can increase the cytotoxic effect.Conclusions: Our results underline the importance of in vitro cytotoxicity screening that should be considered mandatory before performing studies aimed to evaluate the effect of bioactives on other cellular parameters. Although this study is far from the demonstration of a toxic effect of the tested bioactives when administered to humans, it represents a starting point for future research aimed at verifying the existence of a potential hazard due to the wide use of high doses of multiple bioactives. |
| وصف الملف: | ELETTRONICO |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd25f1b7df3dab7a85a90c866a8bfaae https://zenodo.org/record/888419 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dd25f1b7df3dab7a85a90c866a8bfaae |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |