Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice
| العنوان: | Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice |
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| المؤلفون: | Benjamin Mathieu, Annick Ayon, Dongqing Shi, Benjamin Kim, Vincent Villette, Abdelali Jalil, Stéphane Dieudonné, Lagnajeet Pradhan, Simon Chamberland, Renzhi Yang, Michael Z. Lin, Jun B. Ding, Jonathan Bradley, François St-Pierre, Katalin Tóth, Mark J. Schnitzer, Mariya Chavarha, Ivan Dimov, Guo-Qiang Bi, Stephen W Evans |
| المساهمون: | Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Cell Cell, Elsevier, 2019, 179 (7), pp.1590-1608.e23. ⟨10.1016/j.cell.2019.11.004⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, [SDV]Life Sciences [q-bio], Green Fluorescent Proteins, Action Potentials, CHO Cells, Biology, General Biochemistry, Genetics and Molecular Biology, Running, Rats, Sprague-Dawley, Mice, 03 medical and health sciences, Responsivity, Cricetulus, 0302 clinical medicine, Two-photon excitation microscopy, Cricetinae, medicine, Animals, Humans, Theta Rhythm, Wakefulness, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, business.industry, Subthreshold conduction, GTPase-Activating Proteins, Brain, Phosphoric Monoester Hydrolases, Rats, Mice, Inbred C57BL, Optogenetics, Electrophysiology, HEK293 Cells, Microscopy, Fluorescence, Multiphoton, Visual cortex, medicine.anatomical_structure, Modulation, Optical recording, Optoelectronics, Female, business, Ultrashort pulse, 030217 neurology & neurosurgery |
| الوصف: | Summary Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased stability and sensitivity. Combining a soma-targeted ASAP3 variant and ULoVE, we show single-trial tracking of spikes and subthreshold events for minutes in deep locations, with subcellular resolution and with repeated sampling over days. In the visual cortex, we use soma-targeted ASAP3 to illustrate cell-type-dependent subthreshold modulation by locomotion. Thus, ASAP3 and ULoVE enable high-speed optical recording of electrical activity in genetically defined neurons at deep locations during awake behavior. |
| اللغة: | English |
| تدمد: | 0092-8674 1097-4172 |
| DOI: | 10.1016/j.cell.2019.11.004⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcb96fb4538b076cf8ff375b76404095 https://hal.archives-ouvertes.fr/hal-02998862/document |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dcb96fb4538b076cf8ff375b76404095 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00928674 10974172 |
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| DOI: | 10.1016/j.cell.2019.11.004⟩ |