Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase
| العنوان: | Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase |
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| المؤلفون: | Bohdan Vus, Yeshayahu Nitzan, Oleh Smutok, Marina Nisnevitch, Mykhailo Gonchar, Galina Gayda, Sasi Sigawi, Olha Demkiv |
| المصدر: | Materials Materials, Vol 7, Iss 2, Pp 1055-1068 (2014) Materials; Volume 7; Issue 2; Pages: 1055-1068 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Analyte, Spectrum analyzer, Working electrode, Formaldehyde, Analytical chemistry, Horseradish peroxidase, lcsh:Technology, Article, Hansenula polymorpha, chemistry.chemical_compound, General Materials Science, biosensor, alcohol oxidase, lcsh:Microscopy, lcsh:QC120-168.85, Chromatography, formaldehyde, microcomputer-based analyzer, chemosensing electrode, biology, lcsh:QH201-278.5, lcsh:T, Amperometry, Alcohol oxidase, chemistry, lcsh:TA1-2040, biology.protein, lcsh:Descriptive and experimental mechanics, lcsh:Electrical engineering. Electronics. Nuclear engineering, lcsh:Engineering (General). Civil engineering (General), Biosensor, lcsh:TK1-9971 |
| الوصف: | A laboratory prototype of a microcomputer-based analyzer was developed for quantitative determination of formaldehyde in liquid samples, based on catalytic chemosensing elements. It was shown that selectivity for the target analyte could be increased by modulating the working electrode potential. Analytical parameters of three variants of the amperometric analyzer that differed in the chemical structure/configuration of the working electrode were studied. The constructed analyzer was tested on wastewater solutions that contained formaldehyde. A simple low-cost biosensor was developed for semi-quantitative detection of airborne formaldehyde in concentrations exceeding the threshold level. This biosensor is based on a change in the color of a solution that contains a mixture of alcohol oxidase from the yeast Hansenula polymorpha, horseradish peroxidase and a chromogen, following exposure to airborne formaldehyde. The solution is enclosed within a membrane device, which is permeable to formaldehyde vapors. The most efficient and sensitive biosensor for detecting formaldehyde was the one that contained alcohol oxidase with an activity of 1.2 U·mL−1. The biosensor requires no special instrumentation and enables rapid visual detection of airborne formaldehyde at concentrations, which are hazardous to human health. |
| وصف الملف: | application/pdf |
| تدمد: | 1996-1944 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da6f691c1bd0c58c501d29fb38a1d17d https://pubmed.ncbi.nlm.nih.gov/28788499 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....da6f691c1bd0c58c501d29fb38a1d17d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19961944 |
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