Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1
| العنوان: | Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1 |
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| المؤلفون: | Riikka Ihalin, Maribasappa Karched, Casey Chen, Deyu Zhong, Sirkka Asikainen |
| المصدر: | Medical Microbiology and Immunology |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Flp1, genetic structures, Serotypes, Cell, Fimbria, CHOP, Aggregatibacter actinomycetemcomitans, immune system diseases, hemic and lymphatic diseases, polycyclic compounds, Immunology and Allergy, Original Investigation, biology, Chemistry, General Medicine, Blood, medicine.anatomical_structure, Pasteurellaceae Infections, Microbiology (medical), Blood Bactericidal Activity, medicine.drug_class, Phosphorylcholine, Protein subunit, Immunoblotting, 030106 microbiology, Immunology, Serogroup, Monoclonal antibody, Microbiology, C-reactive protein, Fimbriae, 03 medical and health sciences, Bacterial Proteins, medicine, Humans, Periodontitis, Mouth, Microbial Viability, Serum resistance, Proteinase K, biology.organism_classification, Molecular biology, eye diseases, Mikrobiologi, Microscopy, Fluorescence, biology.protein, Gene Deletion |
| الوصف: | Phosphorylcholine (ChoP) is covalently incorporated into bacterial surface structures, contributing to host mimicry and promoting adhesion to surfaces. Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which surface structures bear ChoP, and whether ChoP-positivity relates to serum killing. The tested oral (N = 67) and blood isolates (N = 27) represented 6 serotypes. Mab TEPC-15 was used for immunoblotting of cell lysates and fractions and for immunofluorescence microscopy of cell surface-bound ChoP. The lysates were denatured with urea for hidden ChoP or treated with proteinase K to test whether it binds to a protein. Three ChoP-positive and two ChoP-negative strains were subjected to serum killing in the presence/absence of CRP and using Ig-depleted serum as complement source. Cell lysates and the first soluble cellular fraction revealed a |
| وصف الملف: | application/pdf |
| تدمد: | 1432-1831 0300-8584 |
| DOI: | 10.1007/s00430-018-0554-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9ad42d3e5ea791ec947bc16a409ef5f https://doi.org/10.1007/s00430-018-0554-1 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d9ad42d3e5ea791ec947bc16a409ef5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321831 03008584 |
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| DOI: | 10.1007/s00430-018-0554-1 |