Phenotypic and genotypic characterization of peptidoglycan hydrolases of [i]Lactobacillus sakei[/i]
| العنوان: | Phenotypic and genotypic characterization of peptidoglycan hydrolases of [i]Lactobacillus sakei[/i] |
|---|---|
| المؤلفون: | Monique Zagorec, Stéphane Chaillou, Diego Mora, Hadda Ouzari, Afef Najjari, Houda Amairi, Abdellatif Boudabous |
| المساهمون: | Faculté des Sciences de Tunis, LR03ES03 Laboratoire de Microbiologie et Biomolécules Actives, Université Tunis El Manar (UTM), Université de Tunis, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche Flore Lactique et Environnement Carné (UFLEC), Institut National de la Recherche Agronomique (INRA), Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli studi di Milano [Milano], UMR 1014 SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-Département Microbiologie et Chaîne Alimentaire (MICA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-SECurité des ALIments et Microbiologie (SECALIM), Najjari, Afef, Université de Tunis El Manar (UTM), Università degli Studi di Milano [Milano] (UNIMI), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS) |
| المصدر: | Journal of Advanced Research Journal of Advanced Research, 2015, ⟨10.1016/j.jare.2015.04.004⟩ Journal of Advanced Research 1 (7), 155-163. (2016) Journal of Advanced Research, 2015, 7 (1), ⟨10.1016/j.jare.2015.04.004⟩ Journal of Advanced Research, Vol 7, Iss 1, Pp 155-163 (2016) |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Autolysis (biology), conservateur biologique, peptidoglycan hydrolase, bactérie lactique, [SDV]Life Sciences [q-bio], 030106 microbiology, autolysis, lactobacillus sakei, medicine.disease_cause, Amidase, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, mutanolysin, Listeria monocytogenes, medicine, LysM domains, lcsh:Science (General), General, fermentation, 2. Zero hunger, lcsh:R5-920, Multidisciplinary, biology, food and beverages, biology.organism_classification, Lactobacillus sakei, chemistry, Biochemistry, Listeria, Fermentation, Original Article, Peptidoglycan, Listeria ivanovii, lcsh:Medicine (General), lcsh:Q1-390 |
| الوصف: | Article in press; [i]Lactobacillus sakei[/i], a lactic acid bacterium naturally found in fresh meat and sea products, isconsidered to be one of the most important bacterial species involved in meat fermentation and bio-preservation. Several enzymes of Lb. sakei species contributing to microbial safeguarding and organoleptic properties of fermented-meat were studied. However, the specific autolytic mechanisms and associated enzymes involved in Lb. sakei are not well understood. The autolytic phenotype of 22 Lb. sakei strains isolated from Tunisian meat and seafood products was evaluated under starvation conditions, at pH 6.5 and 8.5, and in the presence of different carbonsources. A higher autolytic rate was observed when cells were grown in the presence of glucose and incubated at pH 6.5. Almost all strains showed high resistance to mutanolysin, indicating a minor role of muramidases in Lb. sakei cell lysis. Using Micrococcus lysodeikticus cells as a substrate in activity gels zymogram, peptidoglycan hydrolase (PGH) patterns for all strains was characterized by two lytic bands of 80 (B1) and 70 kDa (B2), except for strain BMG.167which harbored two activity signals at a lower MW. Lytic activity was retained in high salt and in acid/basic conditions and was active toward cells of Lb. sakei, Listeria monocytogenes, Listeria ivanovii and Listeria innocua. Analysis of five putative PGH genes found in the Lb. sakei 23 K model strain genome, indicated that one gene, lsa1437, could encode a PGH (Nacetylmuramoyl-L-alanine amidase) containing B1 and B2 as isoforms. According to this hypothesis, strain BMG.167 showed an allelic version of lsa1437 gene deleted of one of the five LysM domains, leading to a reduction in the MW of lytic bands and the high autolytic rate of this strain. Characterization of autolytic phenotype of Lb. sakei should expand the knowledgeof their role in fermentation processes where they represent the dominant species. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2090-1232 |
| DOI: | 10.1016/j.jare.2015.04.004⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9098bf34836ef582f6338c6abd50924 https://hal.archives-ouvertes.fr/hal-01204469 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d9098bf34836ef582f6338c6abd50924 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20901232 |
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| DOI: | 10.1016/j.jare.2015.04.004⟩ |