Datura innoxia plants hydroponically-inoculated with Agrobacterium rhizogenes display an enhanced growth and alkaloid metabolism
| العنوان: | Datura innoxia plants hydroponically-inoculated with Agrobacterium rhizogenes display an enhanced growth and alkaloid metabolism |
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| المؤلفون: | Michèle Boitel-Conti, Séverine Schiltz, Armand Guckert, Thi Dao Vu, Christophe Robin, Julie Moussaron, Laure-Anne Bouquet, Thi Kieu Oanh Nguyen, Eric Gontier, Flore Biteau, Alain Hehn, Frédéric Bourgaud, Cyril Jousse, Thi Le Minh Tran, Corinne Assaf-Ducrocq, Nathalie Pawlicki-Jullian |
| المساهمون: | Laboratoire Agronomie et Environnement (LAE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV), SIGMA Clermont (SIGMA Clermont), Vietnam Academy of Science and Technology (VAST), Nong Lam University [Hô-Chi-Minh], Hôpital d'Instruction des Armées du Val de Grâce, Service de Santé des Armées, Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Nong Lam University [Hô-Chi-Minh] (NLU), Biologie des Plantes et Innovation (BIOPI), Sigma CLERMONT (Sigma CLERMONT) |
| المصدر: | Plant Science Plant Science, Elsevier, 2018, 277, pp.166-176. ⟨10.1016/j.plantsci.2018.09.001⟩ Plant Science, 2018, 277, pp.166-176. ⟨10.1016/j.plantsci.2018.09.001⟩ Plant Science, Elsevier, 2018, ⟨10.1016/j.plantsci.2018.09.001⟩ |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Agrobacterium, Plant Development, GUS reporter system, Plant Science, 01 natural sciences, 03 medical and health sciences, Alkaloids, Hydroponics, Semi-composite plant, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Botany, Genetics, medicine, Axenic, ComputingMilieux_MISCELLANEOUS, Hyoscyamine, Plant secondary metabolism, Chimeric plant, 2. Zero hunger, biology, fungi, Tropane alkaloids, food and beverages, General Medicine, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, 15. Life on land, biology.organism_classification, Agrobacterium rhizogenes, 030104 developmental biology, Datura innoxia, Datura, Subculture (biology), [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Agronomy and Crop Science, 010606 plant biology & botany, medicine.drug |
| الوصف: | International audience; Background: The production of secondary metabolites through the culture of entire plants is of great interest. Soilless culture, such as hydroponics, enables the control of plant growth and metabolism. Specific environmental conditions must be developed to maximize the productivity of medicinal plants used as efficient natural bioreactors. Methods: The nutrient solution of newly established hydroponic cultures ofDatura innoxia Mill. were inoculated with Agrobacterium rhizogenes (A.r.) wild strains (TR7, TR107, 11325 or 15834). Growth and the alkaloid contents of roots and aerial parts were analyzed. Axenic cultures were also performed with modified TR7 strains containing the egfp or gus reporter gene. In vitro isolated root cultures enabled the phenological and molecular demonstration of gene transfer. Results: A.r.TR 7 led to a greater improvement in plant secondary metabolism and growth. Positive expression of the reporter genes occurred. Isolation and subculture of some of the roots of these plants showed a hairy root phenotype; molecular tests proved the transfer of bacterial genes into the roots isolated from the plants. Conclusions: Hyoscyamine and scopolamine productivity is enhanced after A.r. inoculation in the nutrient solution of hydroponic plants. Transformation events occur in the original roots of the plants. This leads to chimeric plants with a part of their roots harboring a hairy root phenotype. Such semi-composite plants could be used for successful specialized metabolite bioproduction in greenhouses. |
| تدمد: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2018.09.001 |
| DOI: | 10.1016/j.plantsci.2018.09.001⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8aa2b00f7efb56219aafa7d6882e5f4 https://doi.org/10.1016/j.plantsci.2018.09.001 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d8aa2b00f7efb56219aafa7d6882e5f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01689452 |
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| DOI: | 10.1016/j.plantsci.2018.09.001 |