The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression
| العنوان: | The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression |
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| المؤلفون: | Carl L. Schildkraut, Marc Delepine, Christophe Chevillard, Nasrin Ashouian, Roy Riblet, Fumihiko Matsuda, Jie Zhou, Barbara K. Birshtein |
| المساهمون: | Department of Cell Biology [Bronx, NY, USA], Albert Einstein College of Medicine [New York], Centre National de Génotypage (CNG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Torrey Pines Institute for Molecular Studies, Spinelli, Lionel |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2002, 99 (21), pp.13693-13698. ⟨10.1073/pnas.212392399⟩ Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 (21), pp.13693-13698. ⟨10.1073/pnas.212392399⟩ |
| بيانات النشر: | The National Academy of Sciences, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | DNA Replication, Chromosomes, Artificial, Bacterial, Molecular Sequence Data, Repressor, chemical and pharmacologic phenomena, Replication Origin, Biology, Origin of replication, Histone Deacetylases, Cell Line, 03 medical and health sciences, Mice, 0302 clinical medicine, Immunoglobulin heavy chain (IgH), immune system diseases, hemic and lymphatic diseases, Transcriptional regulation, Animals, Replicon, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, 030304 developmental biology, Genetics, Regulation of gene expression, Expressed Sequence Tags, 0303 health sciences, Expressed sequence tag, Plasma cells, Multidisciplinary, CCAAT-Enhancer-Binding Protein-beta, DNA replication, Proteins, hemic and immune systems, Biological Sciences, LIM Domain Proteins, Immunoglobulin A, Repressor Proteins, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Gene Expression Regulation, Murine erythroleukemia (MEL), Multigene Family, Trans-Activators, Immunoglobulin heavy chain, Carrier Proteins, Immunoglobulin Heavy Chains, 030215 immunology |
| الوصف: | The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murineIghgene cluster. We report here that the nearest non-Ighgenes—Crip,Crp2, andMta1—are located ≈70 kb further downstream and are beyond the end of the domain ofIghtranscriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′Ighregulatory region andCrip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C,-D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of theIghregulation domain. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.212392399⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d76a83a794527f4e3166dbf5c10b7f20 https://europepmc.org/articles/PMC129745/ |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d76a83a794527f4e3166dbf5c10b7f20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.212392399⟩ |