A Small Animal Model for Mother-to-Fetus Transmission of ts1, a Murine Retrovirus
| العنوان: | A Small Animal Model for Mother-to-Fetus Transmission of ts1, a Murine Retrovirus |
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| المؤلفون: | Henry Okonta, Stacie Clark, Joan Duggan, Joana Chakraborty |
| المصدر: | Viral Immunology. 16:191-201 |
| بيانات النشر: | Mary Ann Liebert Inc, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Male, Placenta, Immunology, Human immunodeficiency virus (HIV), HIV Infections, Biology, medicine.disease_cause, Polymerase Chain Reaction, Viral infection, Virus, Mice, Retrovirus, Pregnancy, Virology, Small animal, medicine, Animals, Humans, Pregnancy Complications, Infectious, Mice, Inbred BALB C, Fetus, Transmission (medicine), Neurodegenerative Diseases, biology.organism_classification, Infectious Disease Transmission, Vertical, Disease Models, Animal, Microscopy, Electron, Molecular Medicine, Female, Reproductive capacity, Moloney murine leukemia virus, Spleen, Retroviridae Infections |
| الوصف: | Infection with a murine retrovirus, MoMuLV-TB, ts1 in BALB/c mice has been established as a small animal model for retroviral neurodegenerative disease as shown with infections such as HIV. However, mother-to-pup transmission has never been demonstrated in this model. The current investigation examines vertical transmission of ts1 in this mouse model. A total of 15 females were used to produce 59 pups (16 were used for control, and 43 were used as experimental animals). For experiment 1, 24 5-day-old mice were injected with [0.2 mL of 2.0 x 10(6) ffu/mL ts1] virus. For experiment 2, 19 48-h-old mice were injected with [0.1 mL of 4 x 10(6) ffu/mL ts1] virus. Control groups were injected with DMEM only. PCR and electron microscopy were performed to determine the presence of virus. All mice from experiment 1 injected with ts1 showed viral infection, and retained 100% reproductive capacity. Three out of 102 pups produced by these infected females were infected with ts1. Nine percent of the pups from experiment 2 injected with ts1 retained normal reproductive capacity, and two out of eight (25%) pups had viral infection. Vertical transmission of this unique retrovirus occurs and is dependent, in part, on the timing of maternal infection. |
| تدمد: | 1557-8976 0882-8245 |
| DOI: | 10.1089/088282403322017929 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d75ae57bc499498763553f66a589ded1 https://doi.org/10.1089/088282403322017929 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d75ae57bc499498763553f66a589ded1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578976 08828245 |
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| DOI: | 10.1089/088282403322017929 |