Extending an in vitro panel for estrogenicity testing: the added value of bioassays for measuring antiandrogenic activities and effects on steroidogenesis
| العنوان: | Extending an in vitro panel for estrogenicity testing: the added value of bioassays for measuring antiandrogenic activities and effects on steroidogenesis |
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| المؤلفون: | Harrie Besselink, Si Wang, Jeroen C.W. Rijk, Toine F.H. Bovee, Ivonne M.C.M. Rietjens, René Houtman, Abraham Brouwer, Ad A. C. M. Peijnenburg |
| المصدر: | Toxicological sciences 141 (2014) 1 Toxicological sciences, 141(1), 78-89 |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Novel Foods & Agrochains, BU Toxicologie, Protein Array Analysis, Endocrine Disruptors, Pharmacology, Biology, thyroid-function, Novel Foods & Agroketens, Ligands, Toxicology, cell-line, Sensitivity and Specificity, transcriptional activities, Genes, Reporter, In vivo, Cell Line, Tumor, androgen receptor, High-Throughput Screening Assays, Androgen Receptor Antagonists, Humans, CALUX, BU Toxicology, Novel Foods & Agrochains, Toxicologie, VLAG, Reporter gene, Dose-Response Relationship, Drug, Ligand binding assay, BU Toxicology, Estrogen Antagonists, Estrogen Receptor alpha, In vitro toxicology, wistar rats, environmental chemicals, calux method, bisphenol-a, BU Toxicologie, Novel Foods & Agroketens, Biochemistry, Receptors, Androgen, coregulator binding assay, Biological Assay, Thyroid function, pubertal development, Rikilt B&T Toxicologie en Effectanalyse, Estrogen receptor alpha, hormones, hormone substitutes, and hormone antagonists, Protein Binding |
| الوصف: | In the present study, a previously established integrated testing strategy (ITS) for in vitro estrogenicity testing was extended with additional in vitro assays in order to broaden its sensitivity to different modes of action resulting in apparent estrogenicity, i.e., other than estrogen receptor (ER) binding. To this end, an extra set of 10 estrogenic compounds with modes of action in part different from ER binding, were tested in the previously defined ITS, consisting of a yeast estrogen reporter gene assay, an U2OS ERa CALUX reporter gene assay and a cell-free coregulator binding assay. Two androgen reporter gene assays and the enhanced H295R steroidogenesis assay were added to that previous defined ITS. These assays had added value, as several estrogenic model compounds also elicited clear and potent antiandrogenic properties and in addition also showed effects on steroidogenesis that might potentiate their apparent estrogenic effects in vivo. Adding these assays, examining mechanisms of action for estrogenicity apart from ERa binding, gives a more complete and comprehensive assessment of the ability of test compounds to interfere with endocrine signaling. It was concluded that the extended ITS will go beyond in vivo estrogenicity testing by the uterotrophic assay, thereby contributing to the 3R-principles. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1096-6080 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6f96259c5d9d1483239c503196cf87a https://research.wur.nl/en/publications/extending-an-in-vitro-panel-for-estrogenicity-testing-the-added-v |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d6f96259c5d9d1483239c503196cf87a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10966080 |
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