Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope
| العنوان: | Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope |
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| المؤلفون: | Evelyne Goillot, Juan Elezgaray, Alain Arneodo, Françoise Argoul, Francesca Ratti, Lotfi Berguiga, C. Martinez Torres, Laura Streppa, E. Boyer Provera, Laurent Schaeffer |
| المساهمون: | École normale supérieure - Lyon (ENS Lyon), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ondes et Matière d'Aquitaine (LOMA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique de l'ENS Lyon (Phys-ENS), École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, École normale supérieure de Lyon (ENS de Lyon), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Conference on Plasmonics in Biology and Medicine XIII Conference on Plasmonics in Biology and Medicine XIII, Apr 2016, San Francisco, CA, United States. pp.97240G (1-10), ⟨10.1117/12.2211331⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Materials science, Microscope, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Physics::Optics, Dielectric polarisation, Near and far field, 02 engineering and technology, Surface plasmons, 01 natural sciences, law.invention, Quantitative Biology::Cell Behavior, 010309 optics, Optics, law, 0103 physical sciences, Microscopy, Astronomical interferometer, Surface plasmon resonance, Plasmon, Polarisation, business.industry, Interferometers, Surface plasmon, 021001 nanoscience & nanotechnology, Numerical aperture, Heterodyning, Dielectrics, 0210 nano-technology, business |
| الوصف: | International audience; We introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer provides both enhanced sensitivity and long term stability. This microscope takes advantage of the plasmon resonance excitation and the amplification of the electromagnetic field in near-field distance to the gold coated coverslip. This plasmon enhanced evanescent wave microscopy is particularly attractive for the study of cell adhesion and motility since it can be operated without staining of the biological sample. We show that this microscope allows very long-term imaging of living samples, and that it can capture and follow the temporal deformation of C2C12 myoblast cell protusions (lamellipodia), during their migration on a at surface. |
| اللغة: | English |
| DOI: | 10.1117/12.2211331⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d677489431492d85fac39a37e90d8b35 https://hal.archives-ouvertes.fr/hal-01556052 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d677489431492d85fac39a37e90d8b35 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1117/12.2211331⟩ |
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