Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells
| العنوان: | Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells |
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| المؤلفون: | Thomas Gautier, Nelly Fahet, Zohreh Tamanai-Shacoori, Nolwenn Oliviero, Marielle Blot, Aurélie Sauvager, Agnes Burel, Sandrine David-Le Gall, Sophie Tomasi, Sophie Blat, Latifa Bousarghin |
| المساهمون: | Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Microscopie de Rennes (MRic), Université de Rennes (UR)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes - Faculté des sciences pharmaceutiques et biologiques (UR Pharmacie), Université de Rennes (UR), This work was financially supported by the INSERM, France |
| المصدر: | Microorganisms Microorganisms, 2022, 10 (11), pp.2263. ⟨10.3390/microorganisms10112263⟩ Microorganisms; Volume 10; Issue 11; Pages: 2263 |
| بيانات النشر: | HAL CCSD, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Microbiology (medical), peptide tyrosine tyrosine, hormone-producing cells, quadricellular model, gut commensal bacteria, butyrate, proglucagon, Virology, [SDV]Life Sciences [q-bio], Microbiology |
| الوصف: | International audience; The gut microbiota contributes to human health and disease; however, the mechanisms by which commensal bacteria interact with the host are still unclear. To date, a number of in vitro systems have been designed to investigate the host–microbe interactions. In most of the intestinal models, the enteroendocrine cells, considered as a potential link between gut bacteria and several human diseases, were missing. In the present study, we have generated a new model by adding enteroendocrine cells (ECC) of L-type (NCI-H716) to the one that we have previously described including enterocytes, mucus, and M cells. After 21 days of culture with the other cells, enteroendocrine-differentiated NCI-H716 cells showed neuropods at their basolateral side and expressed their specific genes encoding proglucagon (GCG) and chromogranin A (CHGA). We showed that this model could be stimulated by commensal bacteria playing a key role in health, Roseburia intestinalis and Bacteroides fragilis, but also by a pathogenic strain such as Salmonella Heidelberg. Moreover, using cell-free supernatants of B. fragilis and R. intestinalis, we have shown that R. intestinalis supernatant induced a significant increase in IL-8 and PYY but not in GCG gene expression, while B. fragilis had no impact. Our data indicated that R. intestinalis produced short chain fatty acids (SCFAs) such as butyrate whereas B. fragilis produced more propionate. However, these SCFAs were probably not the only metabolites implicated in PYY expression since butyrate alone had no effect. In conclusion, our new quadricellular model of gut epithelium could be an effective tool to highlight potential beneficial effects of bacteria or their metabolites, in order to develop new classes of probiotics. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-2607 |
| DOI: | 10.3390/microorganisms10112263⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5ba8545cf1c768e371336895fc65e5d https://hal.science/hal-03853165 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d5ba8545cf1c768e371336895fc65e5d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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| DOI: | 10.3390/microorganisms10112263⟩ |