Cartilage-Characteristic Matrix Reconstruction by Sequential Addition of Soluble Factors During Expansion of Human Articular Chondrocytes and Their Cultivation in Collagen Sponges
| العنوان: | Cartilage-Characteristic Matrix Reconstruction by Sequential Addition of Soluble Factors During Expansion of Human Articular Chondrocytes and Their Cultivation in Collagen Sponges |
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| المؤلفون: | Stéphanie Claus, Muriel Piperno, Elisabeth Aubert-Foucher, Odile Damour, Nathalie Mayer, Emeline Perrier-Groult, Frédéric Mallein-Gerin, Jérôme E. Lafont, Hanane Chajra |
| المساهمون: | Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Service de rhumatologie, Hospices Civils de Lyon (HCL)-Affiliation nécessaire..., Banque de tissus et de cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon |
| المصدر: | Tissue Engineering Part C: Methods Tissue Engineering Part C: Methods, Mary Ann Liebert, 2012, 18 (2), pp.104-112. ⟨10.1089/ten.TEC.2011.0259⟩ |
| بيانات النشر: | Mary Ann Liebert Inc, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Cartilage, Articular, [SDV]Life Sciences [q-bio], Biomedical Engineering, Bone Morphogenetic Protein 2, Medicine (miscellaneous), Bioengineering, Bone morphogenetic protein, Organ culture, Fibroblast growth factor, Mice, 03 medical and health sciences, Chondrocytes, 0302 clinical medicine, Nude mouse, Tissue engineering, Gene expression, medicine, Animals, Humans, Insulin, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, Aged, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Tissue Engineering, Tissue Scaffolds, biology, Chemistry, Cartilage, Middle Aged, biology.organism_classification, Immunohistochemistry, In vitro, Extracellular Matrix, Cell biology, medicine.anatomical_structure, Gene Expression Regulation, Solubility, Protein Biosynthesis, 030220 oncology & carcinogenesis, Intercellular Signaling Peptides and Proteins, Triiodothyronine, Cattle, Fibroblast Growth Factor 2, Collagen, Chondrogenesis, Biomedical engineering |
| الوصف: | Articular cartilage has a poor capacity for spontaneous repair. Tissue engineering approaches using biomaterials and chondrocytes offer hope for treatments. Our goal was to test whether collagen sponges could be used as scaffolds for reconstruction of cartilage with human articular chondrocytes. We investigated the effects on the nature and abundance of cartilage matrix produced of sequential addition of chosen soluble factors during cell amplification on plastic and cultivation in collagen scaffolds.Isolated human articular chondrocytes were amplified for two passages with or without a cocktail of fibroblast growth factor (FGF)-2 and insulin (FI). The cells were then cultured in collagen sponges with or without a cocktail of bone morphogenetic protein (BMP)-2, insulin, and triiodothyronine (BIT). The constructs were cultivated for 36 days in vitro or for another 6-week period in a nude mouse-based contained-defect organ culture model. Gene expression was analyzed using polymerase chain reaction, and protein production was analyzed using Western-blotting and immunohistochemistry.Dedifferentiation of chondrocytes occurred during cell expansion on plastic, and FI stimulated this dedifferentiation. We found that addition of BIT could trigger chondrocyte redifferentiation and cartilage-characteristic matrix production in the collagen sponges. The presence of FI during cell expansion increased the chondrocyte responsiveness to BIT. |
| تدمد: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2011.0259 |
| DOI: | 10.1089/ten.TEC.2011.0259⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4132cf6fba8e5dca57912c08a382e57 https://doi.org/10.1089/ten.tec.2011.0259 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d4132cf6fba8e5dca57912c08a382e57 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19373392 19373384 |
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| DOI: | 10.1089/ten.tec.2011.0259 |