A multicenter analytical performance evaluation of a multiplexed immunoarray for the simultaneous measurement of biomarkers of micronutrient deficiency, inflammation and malarial antigenemia
| العنوان: | A multicenter analytical performance evaluation of a multiplexed immunoarray for the simultaneous measurement of biomarkers of micronutrient deficiency, inflammation and malarial antigenemia |
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| المؤلفون: | Christina M Fischer, Carine Mapango, K. Ryan Wessells, Sonja Y. Hess, Eleanor Brindle, James L. Graham, Francisco Arredondo, Crystal D Karakochuk, Lorraine Lillis, Eileen Murphy, Mikaela K Barker, Césaire T. Ouédraogo, David S. Boyle, Neal E. Craft, Christine M. Pfeiffer, Rebecca Barney, Pooja Bansil, Peter J. Havel, Jody M. Randolph, Mindy Zhang, Katherine Wander, Ei Xia Mussai |
| المساهمون: | Sebastiani, Guido |
| المصدر: | PLoS ONE, Vol 16, Iss 11 (2021) PloS one, vol 16, iss 11 PLoS ONE PLoS ONE, Vol 16, Iss 11, p e0259509 (2021) |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Physiology, Organic chemistry, Biochemistry, Medicine and Health Sciences, Medicine, Multicenter Studies as Topic, Vitamin A, Immunoassay, Multidisciplinary, biology, medicine.diagnostic_test, Nutritional Deficiencies, Vitamins, C-Reactive Proteins, Micronutrient, Body Fluids, Physical sciences, Chemistry, Blood, Concordance correlation coefficient, C-Reactive Protein, Micronutrient Deficiencies, Engineering and Technology, Anatomy, Research Article, Quality Control, Analyte, General Science & Technology, Coefficient of variation, Science, Thyroglobulin, Blood Plasma, Chemical compounds, Clinical Research, Industrial Engineering, Organic compounds, Nutrition, Soluble transferrin receptor, Inflammation, Ferritin, Retinol binding protein 4, Chromatography, business.industry, Biology and Life Sciences, Protein Complexes, Proteins, Good Health and Well Being, Ferritins, biology.protein, business, Biomarkers, Software |
| الوصف: | A lack of comparative data across laboratories is often a barrier to the uptake and adoption of new technologies. Furthermore, data generated by different immunoassay methods may be incomparable due to a lack of harmonization. In this multicenter study, we describe validation experiments conducted in a single lab and cross-lab comparisons of assay results to assess the performance characteristics of the Q-plex™ 7-plex Human Micronutrient Array (7-plex), an immunoassay that simultaneously quantifies seven biomarkers associated with micronutrient (MN) deficiencies, inflammation and malarial antigenemia using plasma or serum; alpha-1-acid glycoprotein, C-reactive protein, ferritin, histidine-rich protein 2, retinol binding protein 4, soluble transferrin receptor, and thyroglobulin. Validations included repeated testing (n = 20 separately prepared experiments on 10 assay plates) in a single lab to assess precision and linearity. Seven independent laboratories tested 76 identical heparin plasma samples collected from a cohort of pregnant women in Niger using the same 7-plex assay to assess differences in results across laboratories. In the analytical validation experiments, intra- and inter-assay coefficients of variation were acceptable at |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3b5a9f193d60e1a834b605a413d1335 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568126/?tool=EBI |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d3b5a9f193d60e1a834b605a413d1335 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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