l -Lactate generates hydrogen peroxide in purified rat liver mitochondria due to the putativel -lactate oxidase localized in the intermembrane space
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| المؤلفون: | Anna Atlante, Daniela Valenti, Lidia de Bari, Salvatore Passarella |
| المصدر: | FEBS letters 584 (2010): 2285–2290. doi:10.1016/j.febslet.2010.03.038 info:cnr-pdr/source/autori:de Bari L; Valenti D; Atlante A; Passarella S/titolo:L-lactate generates hydrogen peroxide in purified rat liver mitochondria due to the putative L-lactate oxidase localized in the intermembrane space./doi:10.1016%2Fj.febslet.2010.03.038/rivista:FEBS letters (Print)/anno:2010/pagina_da:2285/pagina_a:2290/intervallo_pagine:2285–2290/volume:584 |
| بيانات النشر: | Wiley, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Biophysics, Respiratory chain, Mitochondria, Liver, Dehydrogenase, Biology, Mitochondrion, Biochemistry, Mixed Function Oxygenases, chemistry.chemical_compound, Structural Biology, Pyruvic Acid, Genetics, Animals, Lactic Acid, Rats, Wistar, Hydrogen peroxide, Molecular Biology, Oxidase test, L-Lactate Dehydrogenase, l-Lactate oxidase, Intracellular Membranes, Cell Biology, Hydrogen-Ion Concentration, Peroxisome, NAD, Oxidants, Rats, Mitochondria, Isoenzymes, l-Lactate, chemistry, NAD+ kinase, Intermembrane space |
| الوصف: | In order to ascertain whether and how mitochondria can produce hydrogen peroxide (H(2)O(2)) as a result of L-lactate addition, we monitored H(2)O(2) generation in rat liver mitochondria and in submitochondrial fractions free of peroxisomal and cytosolic contamination. We found that H(2)O(2) is produced independently on the respiratory chain with 1:1 stoichiometry with pyruvate, due to a putative flavine-dependent L-lactate oxidase restricted to the intermembrane space. The L-lactate oxidase reaction shows a hyperbolic dependence on L-lactate concentration and is inhibited by NAD(+) in a competitive manner, being the enzyme different from the L-lactate dehydrogenase isoenzymes as shown by their pH profiles. |
| تدمد: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2010.03.038 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d39e0602bfeb90ae2deb098bc4af0ed4 https://doi.org/10.1016/j.febslet.2010.03.038 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d39e0602bfeb90ae2deb098bc4af0ed4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00145793 |
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| DOI: | 10.1016/j.febslet.2010.03.038 |