Customized peptidoglycan surfaces to investigate innate immune recognition via surface plasmon resonance
| العنوان: | Customized peptidoglycan surfaces to investigate innate immune recognition via surface plasmon resonance |
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| المؤلفون: | Ha T, Le, Elizabeth A, D'Ambrosio, Siavash, Mashayekh, Catherine Leimkuhler, Grimes |
| المصدر: | Methods in Enzymology ISBN: 9780323952675 Methods Enzymol |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Kinetics, Humans, Biosensing Techniques, Peptidoglycan, Surface Plasmon Resonance, Immunity, Innate, Article |
| الوصف: | Glycan-protein interactions facilitate some of the most important biomolecular processes in and between cells. They are involved in different cellular pathways, cell-cell interactions and associated with many diseases, making these interactions of great interest. However, their structural and functional diversity poses great challenges in studying them at the molecular level. Surface plasmon resonance (SPR) technology presents great advantages to study glycan-protein interactions due to its superior sensitivity, ability to monitor real-time interactions, relatively simple data interpretation, and most importantly, direct measurement of binding without a need for fluorescent labeling. Here, another dimensionality of SPR in studying glycan-protein interactions is demonstrated via examples of binding between human innate immune receptors and their bacterial peptidoglycan ligands. In order to best resemble interactions in solution, a novel strategy of tethering the carbohydrate at different positions to the biosensor surface is applied to represent the potential displays of the carbohydrate ligand to the receptor. Subsequent kinetic analysis provides insights into the optimized configuration of peptidoglycan fragments for binding with its receptors. The manuscript contains a "how-to guide" to help with the implementation of these methods in other glycan-protein binding systems. |
| ردمك: | 978-0-323-95267-5 |
| DOI: | 10.1016/bs.mie.2021.12.004 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d39c468028d3e57bd3e9763dd95de653 https://doi.org/10.1016/bs.mie.2021.12.004 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d39c468028d3e57bd3e9763dd95de653 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9780323952675 |
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| DOI: | 10.1016/bs.mie.2021.12.004 |