Frequency of Mutations inrpoBand Codons 315 and 463 ofkatGin Rifampin- and/or Isoniazid-ResistantMycobacterium tuberculosisIsolates from Northeast Mexico
| العنوان: | Frequency of Mutations inrpoBand Codons 315 and 463 ofkatGin Rifampin- and/or Isoniazid-ResistantMycobacterium tuberculosisIsolates from Northeast Mexico |
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| المؤلفون: | Martha Guerrero-Olazarán, Mariá de los Angeles del Bosque-Moncayo, Claudia Maribel Luna-Aguirre, José M. Viader-Salvadó, Jorge Mauricio Reyes-Ruiz, Rolando Tijerina-Menchaca, Ramón Valdez-Leal |
| المصدر: | Microbial Drug Resistance. 9:33-38 |
| بيانات النشر: | Mary Ann Liebert Inc, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Microbiology (medical), Immunology, Antitubercular Agents, Biology, Polymerase Chain Reaction, Microbiology, law.invention, Mycobacterium tuberculosis, Bacterial Proteins, law, Drug Resistance, Bacterial, Isoniazid, polycyclic compounds, medicine, Codon, Mexico, Gene, Polymerase chain reaction, Electrophoresis, Agar Gel, Pharmacology, DNA-Directed RNA Polymerases, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, rpoB, biology.organism_classification, Virology, Peroxidases, Genetic marker, Mutation, Mutation (genetic algorithm), Mutation testing, bacteria, Rifampin, Polymorphism, Restriction Fragment Length, medicine.drug |
| الوصف: | A total of 48 isoniazid (INH)- and rifampin (RIF)-resistant Mycobacterium tuberculosis isolates, 19 INH-resistant isolates, and 9 RIF-resistant isolates were randomly selected and tested for detecting mutations at codons 315 and 463 of katG by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and/or for detecting mutations at a 69-bp region of the rpoB gene by the INNO-LiPA Rif TB assay. Of the 67 INH-resistant isolates tested, 36 (53.7%) showed the mutation at codon 315 of katG; however, none of them showed the mutation at codon 463. The majority of the RIF-resistant samples analyzed (49 of 57, 86.0%) reacted positive with one of the four R-type probes. The R5-pattern (S531L mutation) was the most frequently observed (31 of 57, 54.4%), followed by R4a-pattern (H526Y mutation) 13 isolates (22.8%), R4b-pattern (H526D mutation) 4 isolates (7.0%), and R2-pattern (D516V mutation) 1 isolate (1.8%). Overall, there was agreement between the line probe kit and phenotypic RIF-susceptibility test for 56 (98.2%) of 57 RIF-resistant isolates tested. These results show that the mutation analysis at codon 315 of katG could be used as a screening assay prior to standard susceptibility testing, whereas mutations in the rpoB gene could be used successfully as genetic markers to rapidly detect RIF-resistant M. tuberculosis clinical isolates from northeast Mexico. |
| تدمد: | 1931-8448 1076-6294 |
| DOI: | 10.1089/107662903764736328 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3997f1599e44ff54a010f0f2363e3cc https://doi.org/10.1089/107662903764736328 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d3997f1599e44ff54a010f0f2363e3cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19318448 10766294 |
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| DOI: | 10.1089/107662903764736328 |