Dietary l-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut
| العنوان: | Dietary l-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut |
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| المؤلفون: | Shrinivas Bishu, Harry L. T. Mobley, Malak H Bazzi, Christopher J. Alteri, Mohamad El-Zaatari, Kathryn A. Eaton, Michael Rodrigues, Nobuhiko Kamada, John Y. Kao, Helmut Grasberger, Chiharu Ishii, Tina L. Morhardt, Naohiro Inohara, Hiroko Nagao-Kitamoto, Sho Kitamoto, Atsushi Hayashi, Tatsuki Nishioka, Kenneth W. Simpson, Peter Kuffa, Kohei Sugihara, Belgin Dogan, Nicolas Barnich, Shinji Fukuda, Stephanie D. Himpsl, Mao Miyoshi, Akiyoshi Hirayama |
| المساهمون: | Hiroko Nagao-Kitamoto, Laboratoire d'automatique et de génie des procédés (LAGEP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Clinical Sciences, College of Veterinary Medicine, Cornell University [New York], Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Division of Gastroenterology, Department of Internal Medicine [Ann Arbor], University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, University of Michigan Center for Gastrointestinal Research, Host Microbiome Initiative NIH 5P30DK034933Kenneth Rainin Foundation United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USADK110146DK108901DK119219Crohn's and Colitis Foundation of America Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science Uehara Memorial Foundation University of Michigan Clinical and Translational Science Awards Program Prevent Cancer Foundation Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI)16H0490117H0565418H04805Japan Science & Technology Agency (JST)JPMJPR1537Japan Science & Technology Agency (JST)JPMJER1902AMED-CREST JP19gm1010009Takeda Science Foundation (TSF) Food Science Institute Foundation Universite Clermont Auvergne Inserm U1071 INRA USC-2018 DK094775, Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Nature Microbiology Nature Microbiology, Nature Publishing Group, 2020, 5 (1), pp.116-125. ⟨10.1038/s41564-019-0591-6⟩ Nature Microbiology, 2020, 5 (1), pp.116-125. ⟨10.1038/s41564-019-0591-6⟩ Nature microbiology |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), Immunology, Inflammation, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Article, 03 medical and health sciences, Mice, Enterobacteriaceae, Genetics, medicine, Citrobacter rodentium, Escherichia coli, Serine, Animals, Intestinal Mucosa, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Catabolism, Pathogenic bacteria, Cell Biology, Metabolism, Gene Expression Regulation, Bacterial, biology.organism_classification, Colitis, Specific Pathogen-Free Organisms, Diet, Mice, Inbred C57BL, Metabolic pathway, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Microbial Interactions, medicine.symptom, Metabolic Networks and Pathways |
| الوصف: | International audience; Metabolic reprogramming is associated with the adaptation of host cells to the disease environment, such as inflammation and cancer. However, little is known about microbial metabolic reprogramming or the role it plays in regulating the fitness of commensal and pathogenic bacteria in the gut. Here, we report that intestinal inflammation reprograms the metabolic pathways of Enterobacteriaceae, such as Escherichia coli LF82, in the gut to adapt to the inflammatory environment. We found that E. coli LF82 shifts its metabolism to catabolize L-serine in the inflamed gut in order to maximize its growth potential. However, L-serine catabolism has a minimal effect on its fitness in the healthy gut. In fact, the absence of genes involved in L-serine utilization reduces the competitive fitness of E. coli LF82 and Citrobacter rodentium only during inflammation. The concentration of luminal L-serine is largely dependent on dietary intake. Accordingly, withholding amino acids from the diet markedly reduces their availability in the gut lumen. Hence, inflammation-induced blooms of E. coli LF82 are significantly blunted when amino acids-particularly L-serine-are removed from the diet. Thus, the ability to catabolize L-serine increases bacterial fitness and provides Enterobacteriaceae with a growth advantage against competitors in the inflamed gut. |
| اللغة: | English |
| تدمد: | 2058-5276 |
| DOI: | 10.1038/s41564-019-0591-6⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d24bab86456e603ab6fef67f52ebec18 https://hal.inrae.fr/hal-02541666 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d24bab86456e603ab6fef67f52ebec18 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20585276 |
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| DOI: | 10.1038/s41564-019-0591-6⟩ |