CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
| العنوان: | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
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| المؤلفون: | Juan Carlos Izpisua Belmonte, Daiji Okamura, Keiichiro Suzuki, Jun Wu, Bret R. McNabb, Marcela Vilarino, Joan D Rowe, Y. S. Bogliotti, Insung Park, Pablo J. Ross |
| المصدر: | Scientific reports, vol 7, iss 1 Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-6 (2017) Wu, J; Vilarino, M; Suzuki, K; Okamura, D; Bogliotti, YS; Park, I; et al.(2017). CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs. Scientific Reports, 7(1). doi: 10.1038/s41598-017-08596-5. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/1j65w9mf |
| بيانات النشر: | eScholarship, University of California, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Pluripotent Stem Cells, Swine, Science, Organogenesis, Computational biology, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Genome editing, Genetics, CRISPR, Animals, Humans, Gene, Pancreas, Homeodomain Proteins, Multidisciplinary, Zygote, Cas9, Human Genome, Embryo, Genetic Therapy, Stem Cell Research, Other Physical Sciences, 030104 developmental biology, Trans-Activators, Tissue and Organ Harvesting, PDX1, Medicine, Biochemistry and Cell Biology, Generic health relevance, Stem cell, CRISPR-Cas Systems, 030217 neurology & neurosurgery, Stem Cell Transplantation, Biotechnology |
| الوصف: | Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs. |
| وصف الملف: | application/pdf |
| DOI: | 10.1038/s41598-017-08596-5. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1af72653ee39618dab794200d1c3bce https://escholarship.org/uc/item/1j65w9mf |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1af72653ee39618dab794200d1c3bce |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1038/s41598-017-08596-5. |
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