We describe two cases of XDRPseudomonas aeruginosainfection caused by a strain of public health concern recently associated with a nationwide outbreak of contaminated artificial tears. Both cases were detected through database review of genomes in the Enhanced Detection System for Hospital-Associated Transmission (EDS-HAT), a routine genome sequencing-based surveillance program. We generated a high-quality reference genome for the outbreak strain from one of the case isolates from our center and examined the mobile elements encodingblaVIM-80andblaGES-9carbapenemases. We then used publicly availableP. aeruginosagenomes to explore the genetic relatedness and antimicrobial resistance genes of the outbreak strain.