The Role of Plasma Membrane Aquaporins in Regulating the Bundle Sheath-Mesophyll Continuum and Leaf Hydraulics
| العنوان: | The Role of Plasma Membrane Aquaporins in Regulating the Bundle Sheath-Mesophyll Continuum and Leaf Hydraulics |
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| المؤلفون: | Christophe Maurel, Gilor Kelly, David Granot, Arava Shatil-Cohen, Menachem Moshelion, Yann Boursiac, Ziv Attia, Nir Sade, Stephen Lerner, Adi Yaaran |
| المساهمون: | Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem (HUJ), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institute of Plant Sciences, Agricultural Research Organization-The Volcani Center, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| المصدر: | Journal of Plant Physiology Journal of Plant Physiology, Elsevier, 2014, 166 (3), pp.1609-20. ⟨10.1104/pp.114.248633⟩ |
| بيانات النشر: | HAL CCSD, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Subfamily, Physiology, [SDV]Life Sciences [q-bio], Arabidopsis, Plasma membrane intrinsic proteins (PIPs), Aquaporin, Plant Science, Leaf hydraulic conductivity (Kleaf), Biology, Photosynthesis, Aquaporins, Permeability, Gene Expression Regulation, Plant, microRNA, Botany, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Promoter Regions, Genetic, Gene, Transpiration, Regulation of gene expression, integumentary system, Arabidopsis Proteins, Cell Membrane, fungi, food and beverages, Plant Transpiration, Aquaporins (AQPs), Articles, Vascular bundle, Plants, Genetically Modified, Artificial microRNA (amiRNA), Cell biology, Plant Leaves, Bundle sheath, Mesophyll Cells |
| الوصف: | Our understanding of the cellular role of aquaporins (AQPs) in the regulation of whole-plant hydraulics, in general, and extravascular, radial hydraulic conductance in leaves (Kleaf), in particular, is still fairly limited. We hypothesized that the AQPs of the vascular bundle sheath (BS) cells regulate Kleaf. To examine this hypothesis, AQP genes were silenced using artificial microRNAs that were expressed constitutively or specifically targeted to the BS. MicroRNA sequences were designed to target all five AQP genes from the PLASMA MEMBRANE-INTRINSIC PROTEIN1 (PIP1) subfamily. Our results show that the constitutively silenced PIP1 (35S promoter) plants had decreased PIP1 transcript and protein levels and decreased mesophyll and BS osmotic water permeability (Pf), mesophyll conductance of CO2, photosynthesis, Kleaf, transpiration, and shoot biomass. Plants in which the PIP1 subfamily was silenced only in the BS (SCARECROW:microRNA plants) exhibited decreased mesophyll and BS Pf and decreased Kleaf but no decreases in the rest of the parameters listed above, with the net result of increased shoot biomass. We excluded the possibility of SCARECROW promoter activity in the mesophyll. Hence, the fact that SCARECROW:microRNA mesophyll exhibited reduced Pf, but not reduced mesophyll conductance of CO2, suggests that the BS-mesophyll hydraulic continuum acts as a feed-forward control signal. The role of AQPs in the hierarchy of the hydraulic signal pathway controlling leaf water status under normal and limited-water conditions is discussed. |
| اللغة: | English |
| تدمد: | 0176-1617 |
| DOI: | 10.1104/pp.114.248633⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1867f3ba059b8e6110fff58e75f224d https://hal.archives-ouvertes.fr/hal-01137644 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1867f3ba059b8e6110fff58e75f224d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01761617 |
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| DOI: | 10.1104/pp.114.248633⟩ |