A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
| العنوان: | A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos |
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| المؤلفون: | Jessica Daolio, Daria Morini, Lodovico Parmegiani, Giovanni Battista La Sala, Francesco Capodanno, Gaetano De Feo, Angela Falbo, Lorenzo Aguzzoli, Alessia Nicoli, Maria Teresa Villani, Luca Braglia |
| المصدر: | Reproductive Biology and Endocrinology : RB&E Reproductive Biology and Endocrinology, Vol 17, Iss 1, Pp 1-8 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Blastomeres, lcsh:QH471-489, Pregnancy Rate, Cryoprotectant, Cleavage Stage, Ovum, lcsh:Gynecology and obstetrics, Cryopreservation, Andrology, Cryoprotective Agents, Endocrinology, Pregnancy, Extracellular, lcsh:Reproduction, Humans, Medicine, Vitrification, lcsh:RG1-991, Retrospective Studies, business.industry, Research, Obstetrics and Gynecology, Embryo, Blastomere, Embryo Transfer, Embryo, Mammalian, Embryo transfer, Reproductive Medicine, IVF, Slow-freezing, Female, Warming, business, Live birth, ART, Developmental Biology |
| الوصف: | Background In the absence of international guidelines indicating the usage of vitrification rather than slow-freezing, the study aim was to analyze a large cohort of slow-frozen/thawed embryos to produce a rationale supporting the standardization of IVF cryopreservation policy. Methods This retrospective analysis included 4779 cleavage stage embryos cryopreserved by slow-freezing/thawing from September 2009 to April 2017 at a single Center. Biological and clinical outcomes of three different commercial kits adopted sequentially, i.e. Vitrolife Cleave Kit® from Vitrolife (kit 1) vs. K-SICS-5000 Kit® and K-SITS-5000 Kit® from Cook Medical (kit 2) and Freeze/Thaw 1™ Kit® from Vitrolife (kit 3) were collected and compared in the light of cryoprotectants composition. Results Kit 3 compared to kit 1 and kit 2 showed significantly (P P P P Conclusions A defined concentration of extracellular cryoprotectants in thawing/warming solutions had a beneficial effect on the embryo cryosurvival rate. Results could provide the rationale for the adoption of a single standardized warming protocol. |
| تدمد: | 1477-7827 |
| DOI: | 10.1186/s12958-019-0519-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d111de05693197d630840d357c69e1ea https://doi.org/10.1186/s12958-019-0519-2 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d111de05693197d630840d357c69e1ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14777827 |
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| DOI: | 10.1186/s12958-019-0519-2 |