Isolation and Characterization of Activators of ERK/MAPK from Citrus Plants
| العنوان: | Isolation and Characterization of Activators of ERK/MAPK from Citrus Plants |
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| المؤلفون: | Sono Watanabe, Takashi Yoshida, Yoshiaki Amakura, Morio Yoshimura, Yoshiko Furukawa, Mitsunari Nakajima, Takahiro Fukata, Satoshi Okuyama |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 13, Iss 2, Pp 1832-1845 (2012) International Journal of Molecular Sciences; Volume 13; Issue 2; Pages: 1832-1845 |
| بيانات النشر: | Molecular Diversity Preservation International (MDPI), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Male, 3,5,6,7,8,3′,4′-heptamethoxyflavone, Citrus, MAP Kinase Signaling System, Biology, CREB, Catalysis, Article, Inorganic Chemistry, lcsh:Chemistry, Mice, Animals, Physical and Theoretical Chemistry, Citrus grandis, Rats, Wistar, Protein kinase A, Extracellular Signal-Regulated MAP Kinases, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, Neurons, MK-801, ERK1/2, Kinase, Plant Extracts, Organic Chemistry, Kawachi bankan, Long-term potentiation, General Medicine, Embryo, Mammalian, Computer Science Applications, Cell biology, Rats, Enzyme Activation, Mice, Inbred C57BL, Biochemistry, lcsh:Biology (General), lcsh:QD1-999, Fruit, methoxyflavone, neurons, Synaptic plasticity, biology.protein, Phosphorylation, NMDA receptor |
| الوصف: | Extracellular signal-regulated kinases 1/2 (ERK1/2), components of the mitogen-activated protein kinase (MAPK) signaling cascade, have been recently shown to be involved in synaptic plasticity and in the development of long-term memory in the central nervous system (CNS). We therefore examined the ability of Citrus compounds to activate ERK1/2 in cultured rat cortical neurons, whose activation might have a protective effect against neurodegenerative neurological disorders. Among the samples tested, extracts prepared from the peels of Citrus grandis (Kawachi bankan) were found to have the greatest ability to activate ERK1/2. The active substances were isolated by chromatographic separation, and one of them was identified to be 3,5,6,7,8,3′,4′-heptamethoxyflavone (HMF). HMF significantly induced the phosphorylation of cAMP response element-binding protein (CREB), a downstream target of activated ERK1/2, which appears to be a critical step in the signaling cascade for the structural changes underlying the development of long-term potentiation (LTP). In addition, the administration of HMF into mice treated with NMDA receptor antagonist MK-801 restored the MK-801-induced deterioration of spatial learning performance in the Morris mater-maze task. Taken together, these results suggest that HMF is a neurotrophic agent for treating patients with memory disorders. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d02a32eb6d5569a9ebc59201f3ddc446 http://europepmc.org/articles/PMC3291996 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d02a32eb6d5569a9ebc59201f3ddc446 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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