Derinat Protects Skin against Ultraviolet-B (UVB)-Induced Cellular Damage
| العنوان: | Derinat Protects Skin against Ultraviolet-B (UVB)-Induced Cellular Damage |
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| المؤلفون: | Manabu Sakakibara, Yaw Bin Huang, Hsin-Su Yu, Wen Li Hsu, Ming Wei Lin, Jia Dai Liu, Shian Jang Yan, Mami Noda, Jian He Lu, Tohru Yoshioka, Ching Ying Wu, Ming-Hsien Tsai |
| المصدر: | Molecules Molecules; Volume 20; Issue 11; Pages: 20297-20311 Molecules, Vol 20, Iss 11, Pp 20297-20311 (2015) Volume 20 Issue 11 Pages 20297-20311 |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Keratinocytes, DNA damage, Ultraviolet Rays, Pharmaceutical Science, Gene Expression, Biology, Derinat, UVB, ROS, calcium, TRPCs, Protective Agents, Article, Analytical Chemistry, Cell Line, lcsh:QD241-441, Transient receptor potential channel, Mice, Calcium imaging, lcsh:Organic chemistry, In vivo, Drug Discovery, Cytotoxic T cell, Animals, Humans, Physical and Theoretical Chemistry, TRPC, Skin, chemistry.chemical_classification, Reactive oxygen species, Mice, Inbred BALB C, integumentary system, Organic Chemistry, DNA, Cell biology, Oxidative Stress, chemistry, Chemistry (miscellaneous), Cyclooxygenase 2, Immunology, Molecular Medicine, Reactive Oxygen Species, Intracellular, DNA Damage |
| الوصف: | Ultraviolet-B (UVB) is one of the most cytotoxic and mutagenic stresses that contribute to skin damage and aging through increasing intracellular Ca(2+) and reactive oxygen species (ROS). Derinat (sodium deoxyribonucleate) has been utilized as an immunomodulator for the treatment of ROS-associated diseases in clinics. However, the molecular mechanism by which Derinat protects skin cells from UVB-induced damage is poorly understood. Here, we show that Derinat significantly attenuated UVB-induced intracellular ROS production and decreased DNA damage in primary skin cells. Furthermore, Derinat reduced intracellular ROS, cyclooxygenase-2 (COX-2) expression and DNA damage in the skin of the BALB/c-nu mice exposed to UVB for seven days in vivo. Importantly, Derinat blocked the transient receptor potential canonical (TRPC) channels (TRPCs), as demonstrated by calcium imaging. Together, our results indicate that Derinat acts as a TRPCs blocker to reduce intracellular ROS production and DNA damage upon UVB irradiation. This mechanism provides a potential new application of Derinat for the protection against UVB-induced skin damage and aging. |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d026190a3d844eb5503e6cd6da0faff3 https://pubmed.ncbi.nlm.nih.gov/26569211 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d026190a3d844eb5503e6cd6da0faff3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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